TVH-19, a synthetic peptide, induces mineralization of dental pulp cells in vitro and formation of tertiary dentin in vivo
TVH-19, a synthetic peptide, induces mineralization of dental pulp cells in vitro and formation of tertiary dentin in vivo
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DOI：
10.1016/j.bbrc.2020.10.095
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发表时间：
2021-01-01
影响因子：
3.1
通讯作者：
Zhang, Linglin
中科院分区：
文献类型：
--
作者：
Han, Sili;Peng, Xiu;Zhang, Linglin
据报道，源自成牙相关蛋白活性结构域的功能肽可促进牙齿硬组织再生。本研究的目的是通过体外和体内实验评估人工合成肽 TVH-19 对间接盖髓 (IPC) 中成牙本质细胞分化和第三级牙本质形成的影响。与对照相比，TVH-19 对人牙髓细胞 (hDPC) 的增殖没有任何影响，但显着促进细胞迁移 (p < 0.05)。 TVH-19 处理的 hDPC 表现出比对照组显着更高的碱性磷酸酶 (ALP) 活性和更强的茜素红染色 (ARS) 反应性 (p < 0.05)。 TVH-19 还上调了成牙基因的 mRNA 和蛋白质表达水平。在大鼠中产生 IPC 后，使用显微计算机断层扫描 (Micro-CT)、苏木精和伊红 (HE) 染色以及免疫组织化学染色对牙齿样本进行研究，以研究 TVH-19 的功能。体内结果表明，TVH-19 诱导第三级牙本质的形成，并减少炎症和细胞凋亡，这一点从白细胞介素 6 (IL-6) 和 cleaved-Caspase-3 (CL-CASP3) 的表达下调中可以看出。总的来说，我们的研究结果表明TVH-19诱导hDPC的分化，促进三级牙本质形成，减轻炎症，减少细胞凋亡，表明TVH-19在IPC中的潜在应用。 (C) 2020 由爱思唯尔公司出版
Functional peptides derived from the active domains of odontogenesis-related proteins have been reported to promote dental hard tissue regeneration. The purpose of this study was to evaluate the effects of an artificially synthesized peptide, TVH-19, on odontoblast differentiation and tertiary dentin formation in indirect pulp capping (IPC) using in vitro and in vivo experiments. TVH-19 did not exhibit any effect on the proliferation of human dental pulp cells (hDPCs) but significantly promoted cell migration, compared with the control (p < 0.05). TVH-19-treated hDPCs showed significantly higher alkaline phosphatase (ALP) activity and stronger alizarin red staining (ARS) reactivity than the control group (p < 0.05). TVH-19 also upregulated the mRNA and protein expression levels of odontogenic genes. After generating IPC in rats, the samples of teeth were studied using micro-computed tomography (Micro-CT), hematoxylin & eosin (HE) staining, and immunohistochemical staining to investigate the functions of TVH-19. The in vivo results showed that TVH-19 induced the formation of tertiary dentin, and reduced inflammation and apoptosis, as evident from the downregulated expression of interleukin 6 (IL-6) and cleaved-Caspase-3 (CL-CASP3). Overall, the results of our study suggest that TVH-19 induces differentiation of hDPCs, promotes tertiary dentin formation, relieves inflammation, and reduces apoptosis, indicating the potential applications of TVH-19 in IPC. (C) 2020 Published by Elsevier Inc.