Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing
Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing
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DOI：
10.1631/jzus.b1900520
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发表时间：
2020-06-01
影响因子：
5.1
通讯作者：
Di, He-shuang
中科院分区：
文献类型：
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作者：
He, Wei-hua;Zhai, Xiao-hu;Di, He-shuang
白藜芦醇（3，5，4 '-trihydroxystilbene，RSV）已被广泛应用于哺乳动物细胞中，但其能否应用于猪精液冷冻过程中尚不清楚。本研究观察了猪精液冷冻过程中RSV处理对其抗冻能力、细胞凋亡及可能的凋亡途径的影响。检测冷冻后（有或无RSV处理）精子活力、线粒体膜电位（Δ psi m）、三磷酸腺苷（ATP）含量、末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）阳性凋亡状态以及参与不同凋亡途径的凋亡基因的信使RNA（mRNA）表达水平。结果表明：（1）与新鲜精子相比，冷冻猪精子的活率、正常顶体率和质膜完整率显著降低（P0.05），但其正常顶体率显著提高（57.65%vs.47.00%，P
Resveratrol (3,5,4'-trihydroxystilbene, RSV) has been widely used in mammalian cells, but whether it can be used during freezing boar semen is still unknown. The effects of RSV treatment during boar semen freezing on its anti-freezing ability, apoptosis, and possible apoptotic pathways were observed in this study. Sperm motility, mitochondrial membrane potential (Delta psi m), adenosine triphosphate (ATP) content, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive apoptotic state, and messenger RNA (mRNA) expression levels of apoptotic genes involved in different apoptotic pathways after freezing with or without RSV treatment were tested. The results showed that: (1) Compared with fresh sperm, the motility, normal acrosome rate, and plasma membrane integrity rate of frozen boar sperm decreased significantly (P0.05), but it did significantly improve the normal acrosome rate (57.65% vs. 47.00%,P