Genome-wide analysis of WRKY transcription factors in white pear (Pyrus bretschneideri) reveals evolution and patterns under drought stress.
Genome-wide analysis of WRKY transcription factors in white pear (Pyrus bretschneideri) reveals evolution and patterns under drought stress.
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白梨 (Pyrus bretschneideri) WRKY 转录因子的全基因组分析揭示了干旱胁迫下的进化和模式
DOI：
10.1186/s12864-015-2233-6
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发表时间：
2015-12-24
期刊：
影响因子：
4.4
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Huang X;Li K;Xu X;Yao Z;Jin C;Zhang S
WRKY转录因子是高等植物中最大的蛋白家族之一，其成员含有1 ~ 2个保守的WRKY结构域，大约60个氨基酸残基，WRKYGQK序列后接C2 H2或C2 HC锌指基序。WRKY蛋白在植物发育、生物胁迫和非生物胁迫应答中起着重要作用。梨（Pyrus bretschneideri）是世界上最重要的水果作物之一，经常受到干旱等非生物胁迫的威胁，影响其生长、发育和生产力。虽然梨基因组序列已经公布，很少有人知道梨WRKY转录因子，特别是在全基因组水平上响应干旱胁迫。根据WRKY蛋白的结构特征和系统发育树的拓扑结构，将梨WRKY（PbWRKY）家族分为7个类群（类群1、2a-e和3）。微系统发育分析表明，PbWRKY基因有33个（32%）为串联重复，57个（55.3%）为节段重复. RNA-seq实验数据和实时荧光定量RT-PCR结果表明，不同类群的PbWRKY基因均受到干旱胁迫的诱导，其中2a和3类群主要参与响应干旱胁迫的生物学途径。此外，适应性进化分析检测到一个显着的正选择组3中的Pbr 001425，其表达模式不同于该组中的其他成员。本研究为进一步研究梨WRKYTFs的功能和分子进化奠定了基础，特别是为通过调控PbWRKYs提高梨的抗旱性奠定了基础。
BackgroundWRKY transcription factors (TFs) constitute one of the largest protein families in higher plants, and its members contain one or two conserved WRKY domains, about 60 amino acid residues with the WRKYGQK sequence followed by a C2H2or C2HC zinc finger motif. WRKY proteins play significant roles in plant development, and in responses to biotic and abiotic stresses. Pear (Pyrus bretschneideri) is one of the most important fruit crops in the world and is frequently threatened by abiotic stress, such as drought, affecting growth, development and productivity. Although the pear genome sequence has been released, little is known about the WRKY TFs in pear, especially in respond to drought stress at the genome-wide level.ResultsWe identified a total of 103 WRKY TFs in the pear genome. Based on the structural features of WRKY proteins and topology of the phylogenetic tree, the pear WRKY (PbWRKY) family was classified into seven groups (Groups 1, 2a–e, and 3). The microsyteny analysis indicated that 33 (32 %)PbWRKYgenes were tandemly duplicated and 57 genes (55.3 %) were segmentally duplicated. RNA-seq experiment data and quantitative real-time reverse transcription PCR revealed thatPbWRKYgenes in different groups were induced by drought stress, and Group 2a and 3 were mainly involved in the biological pathways in response to drought stress. Furthermore, adaptive evolution analysis detected a significant positive selection for Pbr001425 in Group 3, and its expression pattern differed from that of other members in this group. The present study provides a solid foundation for further functional dissection and molecular evolution ofWRKYTFs in pear, especially for improving the water-deficient resistance of pear through manipulation of thePbWRKYs.