Protective function of chloroplast 2-cysteine peroxiredoxin in photosynthesis. Evidence from transgenic Arabidopsis
Protective function of chloroplast 2-cysteine peroxiredoxin in photosynthesis. Evidence from transgenic Arabidopsis
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DOI：
10.1104/pp.119.4.1407
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发表时间：
1999-04-01
期刊：
影响因子：
7.4
通讯作者：
Dietz, KJ
中科院分区：
文献类型：
--
作者：
Baier, M;Dietz, KJ
2-半胱氨酸过氧化物还毒素（2-CPs）是一种普遍存在的过氧化物酶，可将细胞毒性烷基过氧化物还原为相应的醇。最近，我们从植物中克隆了2-CP cdna，并将其定性为叶绿体蛋白。为了阐明2-CP在植物代谢中的生理功能，我们在拟南芥中产生了反义突变体。在突变系中，在叶片和植株发育早期出现2-CP缺乏，并最终积累到野生型水平。在2-CP含量降低的年轻突变体中，光合作用受损，D1蛋白、与光系统II相关的光收集蛋白复合物、叶绿体ATP合成酶和核酮糖-1,5-二磷酸羧化酶/加氧酶水平下降。应用蛋白质合成抑制剂林可霉素后，光抑制特别明显。我们得出结论，光合机制在叶片发育过程中需要高水平的2-CP来保护叶片免受氧化损伤，并且2-CP突变体通过2-CP蛋白的积累、受损蛋白的重新合成和替换以及诱导其他抗氧化防御来减少损伤。
2-Cysteine peroxiredoxins (2-CPs) constitute a ubiquitous group of peroxidases that reduce cell-toxic alkyl hydroperoxides to their corresponding alcohols. Recently, we cloned 2-CP cDNAs from plants and characterized them as chloroplast proteins. To elucidate the physiological function of the 2-CP in plant metabolism, we generated antisense mutants in Arabidopsis. In the mutant lines a 2-CP deficiency developed during early leaf and plant development and eventually the protein accumulated to wild-type levels. In young mutants with reduced amounts of 2-CP, photosynthesis was impaired and the levels of D1 protein, the light-harvesting protein complex associated with photosystem II, chloroplast ATP synthase, and ribulose-1,5-bisphosphate carboxylase/oxygenase were decreased. Photoinhibition was particularly pronounced after the application of the protein synthesis inhibitor, lincomycin. We concluded that the photosynthetic machinery needs high levels of 2-CP during leaf development to protect it from oxidative damage and that the damage is reduced by the accumulation of 2-CP protein, by the de novo synthesis and replacement of damaged proteins, and by the induction of other antioxidant defenses in 2-CP mutants.