Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis
Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis
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DOI：
10.1055/s-1999-2914
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发表时间：
1999-10
期刊：
影响因子：
2
通讯作者：
C. Wojczewski;K. Stolze;J. Engels
中科院分区：
文献类型：
--
作者：
C. Wojczewski;K. Stolze;J. Engels
讨论了核苷和寡核苷酸荧光标记的不同策略及其检测技术。比较了合成前和合成后的路线以及自动化固相合成和酶促合成方法。介绍了量身定制的化学合成方法，以满足作为探针的寡核苷酸的广泛应用要求。
: Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre-and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes