Fibrillogenesis of collagen types I, II, and III with small leucine-rich proteoglycans decorin and biglycan
Fibrillogenesis of collagen types I, II, and III with small leucine-rich proteoglycans decorin and biglycan
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DOI：
10.1021/bm0603746
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发表时间：
2006-08-14
影响因子：
6.2
通讯作者：
Worch, Hartmut
中科院分区：
文献类型：
--
作者：
Douglas, Timothy;Heinemann, Sascha;Worch, Hartmut
胶原蛋白已被用作组织工程的支架材料和植入物的涂层材料，以期通过模仿体内的骨细胞外基质来增强骨整合。本研究的目的是比较I型、II型和III型胶原在磷酸盐缓冲液中体外纤维形成过程中结合富含亮氨酸的小分子蛋白多糖(SLRPs)、粘附素和大聚糖的能力。此外，还研究了SLRPs在高离子强度和低离子强度下对纤维形成过程中胶原分子结合比例的影响，以及对胶原纤维形态和纤维形成速度的影响。这三种胶原蛋白的结合能力都比核心蛋白大得多。与I型和III型胶原相比，II型胶原结合了更多的SLRP。核心蛋白粘附素和Biglycan以相似的方式减少了这三种胶原分子结合到纤维中的比例。Biglycan既不影响纤维直径，也不影响纤维形成的速度。核心蛋白粘附素使三种类型的胶原纤维直径均减小。当选择胶原类型和/或SLRP作为组织工程或种植涂层的支架材料时，不同类型的胶原结合SLRP的能力的差异可能具有重要意义。
Collagen has found use as a scaffold material for tissue engineering as well as a coating material for implants with a view to enhancing osseointegration through mimicry of the bone extracellular matrix in vivo. The aim of this study was to compare the collagen types I, II, and III with regard to their ability to bind the small leucine-rich proteoglycans (SLRPs) decorin and biglycan during fibrillogenesis in vitro in phosphate buffer. In addition, the influence of SLRPs on the proportion of collagen molecules incorporated into fibrils during fibrillogenesis in vitro at high and low ionic strength was investigated, as were their effects on the morphology of collagen fibrils and the speed of fibrillogenesis. Considerably more biglycan than decorin was bound by all three collagen types. Collagen II bound significantly more SLRPs in fibrils than collagen I and III. Decorin and biglycan decreased the proportion of collagen molecules of all three collagen types incorporated into fibrils in similar fashion. Biglycan affected neither fibril diameter nor the speed of fibrillogenesis. Decorin reduced the fibril diameter of all three collagen types. The differences in SLRP-binding ability between collagen types could be of significance when selecting collagen type and/or SLRPs as scaffold materials for tissue engineering or implant coatings.