Developmental expression of sorting nexin 3 in the mouse central nervous system
Developmental expression of sorting nexin 3 in the mouse central nervous system
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DOI：
10.1016/j.gep.2010.08.007
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发表时间：
2011-01-01
影响因子：
1.2
通讯作者：
Tanoue, Akito
中科院分区：
文献类型：
--
作者：
Mizutani, Reiko;Nakamura, Kazuaki;Tanoue, Akito
我们之前报道了分选连接蛋白3 (SNX3)，一种属于分选连接蛋白家族的蛋白，调节小鼠N1E-115神经母细胞瘤细胞的神经突生长。snx3基因在小头畸形、小眼症、外指畸形和前突症（MMEP）和智力低下患者中被破坏，表明snx3在发育过程中这些器官的发生中起重要作用。本研究旨在确定snx3 mRNA的表达模式，特别是在小鼠中枢神经系统（CNS）中，从胚胎期到成年期。胚胎日(E) 9.5和胚胎日(E) 10.5小鼠胚胎的全坐位原位杂交显示，snx3 mRNA在前脑、咽弓、眼睛和肢体芽中表达强烈的阳性信号。胚胎和新生儿脑切片原位杂交分析显示，snx3 mRNA主要表达于大脑皮层、海马、梨状皮质、小脑和脊髓。成年后，snx3 mRNA在大脑皮层、海马、梨状皮质和小脑神经元中均有表达。因此，snx3 mRNA在神经发育过程和成人神经组织中表达，提示snx3可能在中枢神经系统的发育和功能中发挥重要作用。(c) 2010 Elsevier B.V.保留所有权利。
We previously reported that sorting nexin 3 (SNX3), a protein belonging to the sorting nexin family, regulates neurite outgrowth in mouse N1E-115 neuroblastoma cells. The snx3 gene is disrupted in patients with microcephaly, microphthalmia, ectrodactyly, and prognathism (MMEP) and mental retardation, demonstrating that SNX3 plays an important role in the genesis of these organs during development. The present study was designed to determine the expression pattern of snx3 mRNA, particularly in the mouse central nervous system (CNS), from the embryonic stage to adulthood. Whole mount in situ hybridization of embryonic day (E) 9.5 and 10.5 mouse embryos revealed strong positive signals for snx3 mRNA in the forebrain, pharyngeal arches, eyes, and limb buds. in situ hybridization analyses of embryonic and neonatal brain sections revealed that snx3 mRNA is mainly expressed in the cerebral cortex, hippocampus, piriform cortex, cerebellum, and spinal cord. In adulthood, the expression of snx3 mRNA is observed in the cerebral cortex, hippocampus, piriform cortex, and cerebellar neurons. Thus, snx3 mRNA is expressed during neural development and in adult neural tissues, suggesting that SNX3 may play an important role in the development and function of the CNS. (c) 2010 Elsevier B.V. All rights reserved.