Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration.
Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration.
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DOI：
10.3892/mmr.2015.4022
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发表时间：
2015-10
影响因子：
3.4
通讯作者：
Jia T
中科院分区：
文献类型：
--
作者：
Hu P;Feng B;Wang G;Ning B;Jia T
本研究旨在利用生物信息学的方法，以基因芯片为基础，探讨椎间盘退变（IDD）的发生机制。从GeneExpressionOmnibus数据库下载GSE 19943和GSE 34095数据集，筛选IDD差异表达基因。使用不同的算法研究microRNA和靶基因之间的相关性。利用京都基因与基因组途径百科全书和基因本体功能富集分析，探讨靶基因的潜在分子机制。在IDD患者的组织中共鉴定了9种差异表达的microRNA，包括3种下调和6种上调的microRNA和850种DEG。构建了两个microRNA对靶基因的调控网络，包括33个上调的microRNA-靶基因对和4个下调的microRNA-靶基因对。某些靶基因已被证明通过各种途径参与IDD进展，包括细胞周期和癌症途径。此外，还鉴定了两种重要的microRNA（microRNA-222和microRNA-589），它们对IDD的发展至关重要，以及它们的靶基因CDKNAB和SMAD 4。总之，构建了一个全面的miRNA靶基因调控网络，这被认为是重要的IDD进展。
The present study aimed to explore the underlying mechanism of the development of intervertebral disc degeneration (IDD) by bioinformatics based on microarray datasets. GSE 19943 and GSE 34095 datasets downloaded from Gene Expression Omnibus data were used to screen the differentially expressed genes (DEGs) in IDD. The correlation between microRNAs and target genes was investigated using different algorithms. The underlying molecular mechanisms of the target genes were then explored using Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology function enrichment analysis. A total of 9 differentially expressed microRNAs, including 3 down- and 6 upregulated microRNAs and 850 DEGs were identified in tissue from patients with IDD. Two regulation networks of the target genes by microRNAs were constructed, including 33 upregulated microRNA-target gene pairs and 4 downregulated microRNA-target gene pairs. Certain target genes had been demonstrated to be involved in IDD progression via various pathways, including in the cell cycle and pathways in cancer. In addition, two important microRNAs (microRNA-222 and microRNA-589) were identified that were pivotal for the development of IDD, and their target genes, CDKNAB and SMAD4. In conclusion, a comprehensive miRNA-target gene regulatory network was constructed, which was found to be important in IDD progression.