GLYCOGEN-SYNTHASE KINASE 3-BETA IS IDENTICAL TO TAU-PROTEIN KINASE-I GENERATING SEVERAL EPITOPES OF PAIRED HELICAL FILAMENTS
GLYCOGEN-SYNTHASE KINASE 3-BETA IS IDENTICAL TO TAU-PROTEIN KINASE-I GENERATING SEVERAL EPITOPES OF PAIRED HELICAL FILAMENTS
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DOI：
10.1016/0014-5793(93)81066-9
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发表时间：
1993-07-05
期刊：
影响因子：
3.5
通讯作者：
IMAHORI, K
中科院分区：
文献类型：
--
作者：
ISHIGURO, K;SHIRATSUCHI, A;IMAHORI, K
我们以前报道，tau蛋白激酶I（TPKI）诱导正常的tau蛋白成状态的成对螺旋丝（PHF），这是进一步证实了在这里使用几种抗体的免疫印迹分析。我们还提出了TPKI的氨基酸序列，这是相同的糖原合成酶激酶3 β（GSK3 β）。此外，我们发现TPKI活性在整个纯化过程中与GSK 3活性密不可分。这些结果表明TPKI与GSK 3 β相同。
We previously reported that tau protein kinase I (TPKI) induced normal tau protein into a state of paired helical filaments (PHF); this is further confirmed here by immunoblot analysis using several antibodies. We also present the amino acid sequence of TPKI, which is identical to glycogen synthase kinase 3beta (GSK3beta). Moreover, we found that TPKI activity was inseparable from GSK3 activity throughout the purification procedure. These results indicate that TPKI is identical to GSK3beta.