Methods of SARS-CoV-2 Inactivation.

Methods of SARS-CoV-2 Inactivation.
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SARS-CoV-2 灭活方法。

DOI:
10.1007/978-1-0716-2111-0_25
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发表时间:
2022
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Anderson ER
Anderson ER
中科院分区:
--
文献类型:
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作者:
Anderson ER

文献摘要

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灭活方法允许在下游实验中处置和安全使用危害类别3(HG3)病原体,并在较低的遏制水平下进行分析。常用的病毒灭活方法包括热灭活法、固定法、紫外线(UV)光灭活法和洗涤剂灭活法。在这里,我们描述了用于安全下游生物检测的用于灭活SARS-CoV-2的已知方法。
Inactivation methods allow for hazard group 3 (HG3) pathogens to be disposed of and used safely in downstream experiments and assays to be carried out at lower containment levels. Commonly used viral inactivation methods include heat inactivation, fixation methods, ultraviolet (UV) light and detergent inactivation. Here we describe known methods used to inactivate SARS-CoV-2 for safe downstream biological assays.