Maintenance of species boundaries in three sympatric Ligularia (Senecioneae, Asteraceae) species
Maintenance of species boundaries in three sympatric Ligularia (Senecioneae, Asteraceae) species
复制标题
维持三种同域橐吾属（千里光科、菊科）物种的物种边界
DOI：
10.1111/jipb.12674
复制
发表时间：
2018
影响因子：
11.4
通讯作者：
Xun Gong
中科院分区：
文献类型：
--
作者：
Ningning Zhang;Yongpeng Ma;Ryan A. Folk;Jiaojun Yu;Yuezhi Pan;Xun Gong
物种形成的关键过程涉及不同谱系之间生殖隔离屏障的形成和维持。尽管在许多分类单元中经常研究两个物种之间的物种界限，但多个物种（>2）之间的生殖隔离障碍仍然是自然界中最常见的现象，有待澄清。在这里，我们使用双消化限制性位点相关DNA（ddRAD）测序检查模式的杂交在同域的网站，三个Ligularia种生长在一起，并验证这些模式是否有助于维护物种之间的边界。基于RAD SNP数据集的结果表明，杂交Ligularia cyathiceps L. duciformis和L.鸭茅属结果表明，云南稻和云南稻均局限于F1代和少量的第一代回交，没有发现基因渗入，杂交种间存在较强的生殖隔离。此外，混合群体模拟，使用HYBRIDLAB，表明RAD SNP数据集有足够的区分能力，准确的混合检测。研究表明，亲种表现出较强的生殖隔离，且仍维持着种间界限，这可能是青藏高原东部及邻区橐吾属植物维持物种多样性的关键机制。此外，这项研究突出了RAD测序在杂交研究中的有效性。
The key process in speciation concerns the formation and maintenance of reproductive isolating barriers between diverging lineages. Although species boundaries are frequently investigated between two species across many taxa, reproductive isolating barriers among multiple species (>2) that would represent the most common phenomenon in nature, remain to be clarified. Here, we use double digest restriction-site associated DNA (ddRAD) sequencing to examine patterns of hybridization at a sympatric site where three Ligularia species grow together and verify whether those patterns contribute to the maintenance of boundaries among species. The results based on the RAD SNP datasets indicated hybridization Ligularia cyathiceps L. duciformis and L. duciformis L. yunnanensis were both restricted to F1s plus a few first-generation backcrosses and no gene introgression were identified, giving rise to strong reproductive isolation among hybridizing species. Moreover, hybrid swarm simulation, using HYBRIDLAB, indicated the RAD SNP datasets had sufficient discriminatory power for accurate hybrid detection. We conclude that parental species show strong reproductive isolation and they still maintain species boundaries, which may be the key mechanism to maintain species diversity of Ligularia in the eastern Qinghai-Tibetan Plateau and adjacent areas..Moreover, this study highlights the effectiveness of RAD sequencing in hybridization studies.