An animal model of visual loss from orbital hemorrhage.
An animal model of visual loss from orbital hemorrhage.
复制标题
眼眶出血导致视力丧失的动物模型。
DOI：
10.1097/00002341-199409000-00011
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发表时间：
1994
影响因子：
2
通讯作者：
Cunningham,D
中科院分区：
文献类型：
--
作者：
Schabdach,DG;Goldberg,SH;Breton,ME;Griffith,JW;Lang,CM;Cunningham,D
眶内出血可自发发生，也可在眼眶或眶周手术或创伤后发生，并可伴有视力丧失或损害。本项目旨在评价与继发于眶内出血的视力损害相关的眼科效应和潜在机制。一个实验性的外科手术被开发来模拟眶内出血。在9只成年非人灵长类动物（NHP）（Macaca arctoides）中，诱导并维持全身麻醉90、120或180分钟持续时间的单侧视力丧失继发于急性眶内容积增加的可逆状态。在基线、实验和随访过程中进行彩色眼底照相、静脉荧光素血管造影、电生理检查和眼压测量。取眼球和视神经进行组织病理学评价。180分钟实验组中的三只动物中的一只在实验程序后6周表现出视神经病变的临床和组织病理学变化。视神经病变是继发于眶内出血的视力丧失的几种病因之一。这项研究提供了一种可靠、安全和可逆的技术来研究非人灵长类动物眶内容积急性增加的影响。所描述的动物模型可用于评估与其他获得性视神经病变中的视觉损害有关的机制。
Intraorbital hemorrhage may arise spontaneously or following orbital or periorbital surgery or trauma and may be associated with visual loss or impairment. This project was designed to evaluate the ophthalmic effects and underlying mechanism (s) associated with visual impairment secondary to intraorbital hemorrhage. An experimental surgical procedure was developed to simulate intraorbital hemorrhage. A reversible state of unilateral visual loss secondary to acutely increased intraorbital volume was induced and maintained under general anesthesia for either 90, 120, or 180 min duration in nine adult nonhuman primates (NHPs)(Macaca arctoides). Color funduscopic photography, iv fluorescein angiography, electrophysiological testing, and tonometry were obtained during baseline, experimental, and follow-up procedures. The globes and optic nerves were obtained for histopathologic evaluation. One of three animals in the 180 min experimental group exhibited clinical and histopathological changes of optic neuropathy 6 weeks after the experimental procedure. Optic neuropathy is one of several proposed etiologies for producing visual loss secondary to intraorbital hemorrhage. This study offers a reliable, safe, and reversible technique to study the effects of acutely increased intraorbital volume in nonhuman primates. The animal model described may be useful for evaluating mechanism (s) involved with visual impairment in other acquired optic neuropathies.