Lipid imaging within the normal rat kidney using silver nanoparticles by matrix-assisted laser desorption/ionization mass spectrometry.
Lipid imaging within the normal rat kidney using silver nanoparticles by matrix-assisted laser desorption/ionization mass spectrometry.
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DOI：
10.1038/ki.2015.3
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发表时间：
2015-07
影响因子：
19.6
通讯作者：
Woods AS
中科院分区：
文献类型：
--
作者：
Muller L;Kailas A;Jackson SN;Roux A;Barbacci DC;Schultz JA;Balaban CD;Woods AS
肾脏的细胞和结构成分特征明确，显示出不同的脂质区域组成和分布。为了更全面地分析肾脂质组，我们开发了一种基质辅助激光解吸/电离质谱成像方法，可用于查明病理条件下正常变化的部位。这是通过使用磁控溅射源植入具有稳定、可定量和可再现的均匀银层的矢状低温恒温器大鼠肾切片以形成银纳米颗粒来实现的。在正离子模式下检测并成像了38种脂质，包括7种神经酰胺、8种二酰基甘油、22种三酰基甘油和胆固醇。由7种鞘磷脂、10种磷脂酰乙醇胺、1种磷脂酰甘油、7种磷脂酰肌醇和11种硫苷脂组成的36种脂质在负离子模式下成像，获得了74张正常肾脏的高分辨率脂质组图。因此，我们的方法是一个强大的工具，不仅用于研究疾病动物模型的结构变化，而且用于诊断和跟踪人类肾脏组织活检中的疾病阶段。
The well-characterized cellular and structural components of the kidney show distinct regional compositions and distribution of lipids. In order to more fully analyze the renal lipidome we developed a matrix-assisted laser desorption/ionization mass spectrometry approach for imaging that may be used to pinpoint sites of changes from normal in pathological conditions. This was accomplished by implanting sagittal cryostat rat kidney sections with a stable, quantifiable and reproducible uniform layer of silver using a magnetron sputtering source to form silver nanoparticles. Thirty-eight lipid species including 7 ceramides, 8 diacylglycerols, 22 triacylglycerols, and cholesterol were detected and imaged in positive ion mode. Thirty-six lipid species consisting of, 7 sphingomyelins, 10 phosphatidylethanolamines, 1 phosphatidylglycerol, 7 phosphatidylinositols and 11 sulfatides, were imaged in negative ion mode for a total of seventy-four high resolution lipidome maps of the normal kidney. Thus, our approach is a powerful tool not only for studying structural changes in animal models of disease, but also for diagnosing and tracking stages of disease in human kidney tissue biopsies.