Landscape of Chimeric RNAs in Non-Cancerous Cells.
Landscape of Chimeric RNAs in Non-Cancerous Cells.
复制标题
非癌细胞中嵌合RNA的图景。
作者：
Chen C;Haddox S;Tang Y;Qin F;Li H
基因融合及其产物（RNA和蛋白质）一直被认为是癌细胞的独特特征，并被用作多种癌症类型的理想生物标志物和药物靶点。然而，最近的研究表明，在正常细胞和组织中也可以发现由基因间选择性剪接产生的嵌合rna。在这项研究中，我们的目的是在不同的非肿瘤细胞系中鉴定嵌合rna，并研究这些新的候选嵌合rna的景观和表达。为此，我们以HEK-293T、HUVEC和LO2细胞系为模型，进行配对端RNA测序，并进行嵌合RNA谱分析。采用多种筛选标准，从多个层面和不同角度对嵌合rna的景观进行了表征。此外，我们通过实验验证了来自不同分类的17种嵌合rna。最后，我们在不同的癌症细胞和非癌症细胞（包括来自健康供者的血液）中检测了许多经过验证的嵌合rna，并证明了它们普遍存在的表达模式。
Gene fusions and their products (RNA and protein) have been traditionally recognized as unique features of cancer cells and are used as ideal biomarkers and drug targets for multiple cancer types. However, recent studies have demonstrated that chimeric RNAs generated by intergenic alternative splicing can also be found in normal cells and tissues. In this study, we aim to identify chimeric RNAs in different non-neoplastic cell lines and investigate the landscape and expression of these novel candidate chimeric RNAs. To do so, we used HEK-293T, HUVEC, and LO2 cell lines as models, performed paired-end RNA sequencing, and conducted analyses for chimeric RNA profiles. Several filtering criteria were applied, and the landscape of chimeric RNAs was characterized at multiple levels and from various angles. Further, we experimentally validated 17 chimeric RNAs from different classifications. Finally, we examined a number of validated chimeric RNAs in different cancer and non-cancer cells, including blood from healthy donors, and demonstrated their ubiquitous expression pattern.
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影响因子：
14.9
作者：
Babiceanu M;Qin F;Xie Z;Jia Y;Lopez K;Janus N;Facemire L;Kumar S;Pang Y;Qi Y;Lazar IM;Li H
通讯作者：
Li H
影响因子：
4.5
作者：
Qin F;Song Z;Babiceanu M;Song Y;Facemire L;Singh R;Adli M;Li H
通讯作者：
Li H
DOI：
10.3324/haematol.2009.011536
发表时间：
2010-01-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
--
作者：
Chase, Andrew;Ernst, Thomas;Cross, Nicholas C. P.
通讯作者：
Cross, Nicholas C. P.
影响因子：
9.7
作者：
Qin F;Song Z;Chang M;Song Y;Frierson H;Li H
通讯作者：
Li H
影响因子：
14.9
作者：
Singh, Sandeep;Qin, Fujun;Li, Hui
通讯作者：
Li, Hui