An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins
An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins
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DOI：
10.1023/a:1006365213954
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发表时间：
2000-01-01
影响因子：
5.1
通讯作者：
Jackowski, G
中科院分区：
文献类型：
--
作者：
Jansson, S;Andersson, J;Jackowski, G
拟南芥cDNA克隆编码一个新的110个氨基酸类囊体蛋白已被测序。体外合成的蛋白质被完整的叶绿体吸收，插入类囊体膜，在此过程中转运肽被切割掉。成熟的蛋白质预计含有69个氨基酸，形成一个跨膜的α -螺旋，其n端位于类囊体膜的基质侧。该蛋白与高等植物的LHC、ELIP和PsbS蛋白相似，但与蓝藻和红藻的高光诱导蛋白（high-light-inducible protein, HLIPs）更为相似，此前未在高等植物中检测到类似蛋白。对于HLIP和ELIP，高光增加了相应基因的mRNA水平。序列比较表明，该蛋白可能与叶绿素结合并在类囊体膜中形成二聚体。该蛋白的表达水平似乎远低于正常PSI和PSII亚基。
An Arabidopsis thaliana cDNA clone encoding a novel 110 amino acid thylakoid protein has been sequenced. The in vitro synthesized protein is taken up by intact chloroplasts, inserted into the thylakoid membrane and the transit peptide is cleaved off during this process. The mature protein is predicted to contain 69 amino acids, to form one membrane-spanning alpha-helix and to have its N-terminus at the stromal side of the thylakoid membrane. The protein showed similarity to the LHC, ELIP and PsbS proteins of higher plants, but more pronounced to the high-light-inducible proteins (HLIPs) of cyanobacteria and red algae, to which no homologue previously has been detected in higher plants. As for HLIP and ELIP, high light increases the mRNA levels of the corresponding gene. Sequence comparisons indicate that the protein may bind chlorophyll and form dimers in the thylakoid membrane. The level of expression of the protein seems to be far lower than that of normal PSI and PSII subunits.