Correlation of APE1 with VEGFA and CD163+macrophage infiltration in bladder cancer and their prognostic significance
Correlation of APE1 with VEGFA and CD163+macrophage infiltration in bladder cancer and their prognostic significance
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膀胱癌中APE1与VEGFA、CD163(+)巨噬细胞浸润的相关性及预后意义
DOI：
10.3892/ol.2020.11814
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发表时间：
2020-09-01
期刊：
影响因子：
2.9
通讯作者：
Jiang, Jun
中科院分区：
文献类型：
--
作者：
Wang, Lin-Ang;Yang, Bo;Jiang, Jun
本研究旨在评估脱嘌呤/脱嘧啶脱氧核糖核酸酶1（APE 1）、血管内皮生长因子A（VEGFA）表达和CD 163（+）肿瘤相关巨噬细胞（TAM）比率作为膀胱癌（BCa）预后因素的适用性。研究共招募了2013年1月至2017年1月在大坪医院接受根治性膀胱切除术的127例膀胱尿路上皮癌患者，其中非肌层浸润性BCa（NMIBC）45例，MIBC 82例。对组织样本进行APE 1、VEGFA和CD 163的免疫组化检测，以及APE 1、VEGFA、CD 163和CD 34的多重免疫荧光染色。对于APE 1和VEGFA，基于强度（0-3）对染色进行分级，而基于阳性染色细胞的百分比对CD 163进行分级（0-3）。采用Kaplan-Meier和考克斯回归分析评估APE 1、VEGF和CD 163的预后价值。结果提示，在BCa中，APE 1高表达与VEGFA高表达和CD 163（+）TAM浸润有关。此外，APE 1的高表达与BCa的淋巴管浸润以及生存时间缩短相关。提示APE 1可能与BCa中CD 163（+）TAM浸润有关，VEGFA可能是其影响因素。
The present study sought to estimate the applicability of apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1), vascular endothelial growth factor A (VEGFA) expression and CD163(+)tumor-associated macrophage (TAM) ratio as prognostic factors in bladder cancer (BCa). A total of 127 patients with bladder urothelial cancer who underwent radical cystectomy at Daping Hospital were recruited between January 2013 and January 2017, including 45 cases of non-muscle invasive BCa (NMIBC) and 82 of MIBC. Immunohistochemical detection of APE1, VEGFA and CD163, as well as multiple immunofluorescence staining for APE1, VEGFA, CD163 and CD34, were performed on tissue samples. For APE1 and VEGFA, the staining was graded based on intensity (0-3), while CD163 was graded (0-3) based on the percentage of positively stained cells. The prognostic value of APE1, VEGF and CD163 was assessed using Kaplan-Meier and Cox regression analysis. The results suggested that in BCa, high APE1 expression was associated with high VEGFA expression and more infiltration of CD163(+)TAM. Furthermore, high expression of APE1 was associated with lymphovascular invasion of BCa, as well as reduced survival time. This indicates that APE1 may be associated with CD163(+)TAM infiltration in BCa, with VEGFA as a possible influencing factor.