Requirement of MHC class I on radioresistant cells for granzyme B expression from CD8+ T cells in murine contact hypersensitivity
Requirement of MHC class I on radioresistant cells for granzyme B expression from CD8+ T cells in murine contact hypersensitivity
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小鼠接触性超敏反应中 CD8 T 细胞表达颗粒酶 B 所需 MHC I 类对放射抗性细胞的要求
DOI：
10.1016/j.jdermsci.2018.01.013
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
and Kenji Kabashima
中科院分区：
文献类型：
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作者：
Sachiko Ono;Tetsuya Honda;and Kenji Kabashima
过敏性接触性皮炎（ACD）是一种常见的皮肤疾病，影响全球约44%-20%的普通人群。半抗原是分子量小于500 Da的45种化合物，被认为与自身肽或46种蛋白质反应，作为ACD中的病原性抗原（Ag）[1，2]。Ag呈递的CD 8 + T 47细胞在激发期产生干扰素（IFN）-r和细胞毒性颗粒，如48颗粒酶B和穿孔素，并引起炎症[2-4]。认为真皮树突状细胞49（dDC）对于将这些半抗原化Ag呈递给CD 8 + T 50细胞至关重要[2，5，6]。另一方面，考虑到半抗原可以调节附着于主要组织相容性复合体（MHC）I类的肽[1]，并且MHC I类52在除dDC之外的多种细胞中表达，已经从一些体外研究中假设ACD中由53辐射抗性细胞进行的dDC非依赖性Ag呈递[7-9]，然而，54其在体内的实际贡献知之甚少。在此，使用鼠接触性超敏反应（CHS），ACD的动物模型，我们56试图阐明在57激发时不依赖dDC的Ag呈递的可能性。58
Allergic contact dermatitis (ACD) is a common cutaneous disorder that affects around 44 15–20% of the general population worldwide. Haptens, chemical compounds of 45 molecular weight smaller than 500 Da, are assumed to react with self-peptides or 46-proteins to serve as pathogenic antigens (Ags) in ACD [1, 2]. Ag-presented-CD8+ T 47 cells at the elicitation phase produce interferon (IFN)-r and cytotoxic granules such as 48 granzyme B and perforin, and provoNe inflammation [2–4]. Dermal dendritic cells 49 (dDCs) are considered essential for the presentation of these haptenized-Ag to CD8+ T 50 cells [2, 5, 6]. On the other hand, given that haptens can modulate peptides that are 51 attached to major histocompatibility complex (MHC) class I [1], and that MHC class I 52 is expressed in a variety of cells other than dDCs, dDC-independent Ag-presentation by 53 radioresistant cells has been assumed in ACD from some in-vitro studies [7–9], however, 54 the actual contribution of which in vivo is poorly understood. 55 Herein, using a murine contact hypersensitivity (CHS), an animal model of ACD, we 56 attempted to clarify the possibility of dDC-independent Ag-presentation at the 57 elicitation. 58