Photoreactivities of 5-Bromouracil-containing RNAs
Photoreactivities of 5-Bromouracil-containing RNAs
复制标题
含 5-溴尿嘧啶 RNA 的光反应性
DOI：
10.1016/j.bmc.2012.11.010
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sugiyama H.
中科院分区：
文献类型：
--
作者：
Morinaga H;Kizaki S;Takenaka T;Kanesato S;Sannohe Y;Tashiro R;Sugiyama H.
5-将溴尿嘧啶（BrU）掺入到三种合成RNA中，用HPLC和MS分析了含BrU的RNA的光辐射产物。A型RNA中的r（GCABrUGC）2和r（CGAABrUUGC）/r（GCAAUUCG）在5′端产生相应的2′-酮腺苷（ketoA）产物，如r（GCketoAUGC）和r（CGAketoAUUGC）。Z型RNA中r（CGCGBrUGCG）/r（CmGCACmGCG）的光照射产生2′-酮基鸟苷（ketoG）产物r（CGCketoGUGCG），而A型RNA中r（CGCGBrUGCG）/r（CmGCACmGCG）的光照射几乎没有产物。目前的结果清楚地表明，光化学产生的尿嘧啶-5-基自由基的氢（H）提取选择性地发生在C2′位，以提供2′-酮RNA产物。
5-Bromouracil (BrU) was incorporated into three types of synthetic RNA and the products of the photoirradiatedBrU-containing RNAs were investigated using HPLC and MS analysis. The photoirradiation of r(GCABrUGC)2and r(CGAABrUUGC)/r(GCAAUUCG) in A-form RNA produced the corresponding 2′-keto adenosine (ketoA) product at the 5′-neighboring nucleotide, such as r(GCketoAUGC) and r(CGAketoAUUGC), respectively. The photoirradiation of r(CGCGBrUGCG)/r(CmGCACmGCG) in Z-form RNA produced the 2′-keto guanosine (ketoG) product r(CGCketoGUGCG), whereas almost no products were observed from the photoirradiation of r(CGCGBrUGCG)/r(CmGCACmGCG) in A-form RNA. The present results indicate clearly that hydrogen (H) abstraction by the photochemically generated uracil-5-yl radical selectively occurs at the C2′ position to provide a 2′-keto RNA product.