Large area two-dimensional B cell arrays for sensing and cell-sorting applications
Large area two-dimensional B cell arrays for sensing and cell-sorting applications
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DOI：
10.1021/bm034341r
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发表时间：
2004-05-01
影响因子：
6.2
通讯作者：
Hammond, PT
中科院分区：
文献类型：
--
作者：
Kim, H;Doh, J;Hammond, PT
利用由新设计的聚烯丙胺-g-聚乙二醇阳离子接枝共聚物组成的微图案化分子模板，制备了大面积规则的非黏附B细胞阵列。聚合物压印(POPS)技术被成功地应用于接枝共聚物在带负电荷的多层表面上的微米级图案，而不会失去对蛋白质的非特异性吸附的阻力。为了生成用于B细胞阵列的模板，通过引入表面生物素化和特异性蛋白质吸附来改变图案化表面的特征。从每个模板得到的B细胞阵列的质量表明，非粘附性B细胞与模板表面之间的结合强度是大面积制备清洁、规则的固定化淋巴细胞阵列的控制因素，这在许多生物技术和免疫学应用中是至关重要的。
Regular arrays of nonadherent B cells over large areas were produced with the use of micropatterned molecular templates consisting of a newly designed poly(allylamine)-g-poly(ethylene glycol) polycation graft copolymer. Polymer-on-polymer stamping (POPS) techniques were applied successfully to create micron scale patterns of the graft copolymer on negatively charged multilayer surfaces without losing resistance to the nonspecific adsorption of proteins. To generate templates for B cell arrays, the characteristics of the patterned surface were modified via introduction of surface biotinylation and specific protein adsorption. The qualities of B cell arrays resulting from each template suggest the binding strength between nonadherent B cells and the template surface is the controlling factor in the fabrication of clean and regular arrays of immobilized lymphocytes over large areas, which is critical in many bio-technological and immunological applications.