Autophagic cell death of human hepatoma G2 cells mediated by procyanidins from Castanea mollissima Bl. Shell-induced reactive oxygen species generation
Autophagic cell death of human hepatoma G2 cells mediated by procyanidins from Castanea mollissima Bl. Shell-induced reactive oxygen species generation
复制标题
板栗原花青素介导的人肝癌 G2 细胞的自噬细胞死亡。
DOI：
10.1016/j.cbi.2014.09.021
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发表时间：
2014-12-05
影响因子：
5.1
通讯作者：
Sun, Xiaobo
中科院分区：
文献类型：
--
作者：
Duan, Yuqing;Ke, Jiajia;Sun, Xiaobo
研究了板栗壳原花青素（CSPCs）诱导人肝癌G2 （HepG2）细胞的自噬作用，并探讨了自噬水平与活性氧（ROS）生成的内在关系。结果表明，CSPCs诱导HepG2细胞呈时间和浓度依赖性死亡，增加自噬溶酶体和微管相关蛋白轻链3-II （LC3-II，自噬标志物）的积累。然而，在自噬抑制剂3-MA预处理组未观察到这些现象，提示CSPCs诱导HepG2细胞自噬。此外，我们发现CSPCs在细胞中触发ROS生成，而n -乙酰半胱氨酸（Nac）共处理时ROS水平下降，表明CSPCs介导的自噬被Nac部分阻断。此外，CSPCs处理降低了HepG2细胞的线粒体膜电位。这些结果表明，CSPCs可能通过ROS的产生引发自噬，这可能与线粒体依赖的信号通路有关。2014爱思唯尔爱尔兰有限公司版权所有。
The autophagy of human hepatoma G2 (HepG2) cells induced by procyanidins from chestnut (Castanea mollissima BI.) shell (CSPCs) was investigated, and the inherent relationship between autophagic levels and reactive oxygen species (ROS) generation was studied. The results showed that CSPCs induced HepG2 cell death in a time- and concentration-dependent manner, increased the accumulation of autophagolysosomes and microtubule-associated proteins light chain 3-II (LC3-II, a marker of autophagy). However, these phenomena were not observed in the group pretreated with the autophagy inhibitor 3-MA, suggesting that CSPCs induced HepG2 cell autophagy. Furthermore, we found that CSPCs triggered ROS generation in cells, while the levels of ROS decreased in the N-acetylcysteine (Nac) co-treatment, revealing that CSPCs-mediated autophagy was partly blocked by Nac. In addition, treatment with CSPCs decreased the mitochondrial membrane potential of HepG2 cells. These results suggested CSPCs could trigger autophagy via ROS generation, which may be associated with the mitochondria-dependent signaling way. (C) 2014 Elsevier Ireland Ltd. All rights reserved.