Differential expression patterns of Nqo1, AKR1B8 and Ho-1 in the liver and small intestine of C57BL/6 mice treated with sulforaphane.
Differential expression patterns of Nqo1, AKR1B8 and Ho-1 in the liver and small intestine of C57BL/6 mice treated with sulforaphane.
复制标题
萝卜硫素处理的 C57BL/6 小鼠肝脏和小肠中 Nqo1、AKR1B8 和 Ho-1 的差异表达模式。
DOI：
10.1016/j.dib.2015.09.029
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发表时间：
2015-12
期刊：
影响因子：
1.2
通讯作者：
Jun Wang X
中科院分区：
文献类型：
--
作者：
Luo L;Chen Y;Wu D;Shou J;Wang S;Ye J;Tang X;Jun Wang X
该数据文章包含与题为“丁基化羟基茴香醚在C57 BL/6小鼠的肝脏和小肠中诱导Nrf 2和解毒酶的不同表达模式”的研究文章相关的补充图和结果（Luo et al.，2015），其定义了C57 BL/6小鼠肝脏和小肠中II期酶Nqo 1、AKR 1B 8和Ho-1的基础和丁基化羟基茴香醚（BHA）诱导的表达模式。萝卜硫素[1-异硫氰酸基-4-（甲基亚磺酰基）丁烷]（SFN）是一种天然存在的异硫氰酸酯，来源于十字花科蔬菜，是一种高效的II相细胞保护酶诱导剂。该数据集报告了SFN诱导的野生型（WT）和Nrf 2-/-小鼠中Nqo 1、AKR 1B 8和Ho-1的组织学变化。给小鼠单次口服25 mg/kg SFN 24 h和48 h。免疫组织化学显示，在WT小鼠的肝脏中，SFN增加了肝细胞中的Nqo 1染色，在中央周围区域的染色略高。AKR 1B 8的诱导主要出现在门静脉周围区域的肝细胞中。基础型和诱导型Ho-1主要位于枯否细胞。在野生型小鼠小肠中，Nqo 1和AKR 1B 8的诱导表达在绒毛中比在隐窝中更明显。
This data article contains complementary figures and results related to the research article entitled “butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice” (Luo et al., 2015), which defined the basal and butylated hydroxyanisole (BHA)-induced expression patterns of Phase II enzymes Nqo1, AKR1B8, and Ho-1 in the liver and small intestine of C57BL/6 mice. Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)butane] (SFN), a naturally occurring isothiocyanate derived from cruciferous vegetables, is a highly potent inducer of phase II cytoprotective enzymes. This dataset reports the histological changes of Nqo1, AKR1B8, and Ho-1 in wild-type (WT) and Nrf2-/- mice induced by SFN. The mice were given a 25 mg/kg single oral dose of SFN for 24 h and 48 h. Immunohistochemistry revealed that, in the liver from WT mice, SFN increased Nqo1 staining in hepatocytes with slight higher staining in the pericentral region. The induction of AKR1B8 appeared mostly in hepatocytes in the periportal region. The basal and inducible Ho-1 was located predominately in Kupffer cells. In the small intestine from WT mice, the inducible expression of Nqo1 and AKR1B8 appeared more obvious in the villus than that in the crypt.