Selective Structural Change by Spermidine in the Bulged-out Region of Double-stranded RNA and Its Effect on RNA Function
Selective Structural Change by Spermidine in the Bulged-out Region of Double-stranded RNA and Its Effect on RNA Function
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DOI：
10.1074/jbc.m806027200
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发表时间：
2008-11-21
影响因子：
4.8
通讯作者：
Igarashi, Kazuei
中科院分区：
文献类型：
--
作者：
Higashi, Kyohei;Terui, Yusuke;Igarashi, Kazuei
多胺主要通过与 RNA 的相互作用在细胞生长中发挥重要作用。我们之前报道过，多胺刺激大肠杆菌中寡肽结合蛋白 OppA 的合成以及大鼠肝脏中 Ile-tRNA 的形成（Igarashi, K. 和 Kashiwagi, K. (2000) Biochem. Biophys. Res. Commun. 271, 559-564)。这些效应涉及多胺与 OppA mRNA 起始区和大鼠肝脏 tRNAIle 受体茎中双链 RNA 凸出区的相互作用。在这项研究中，使用有或没有双链RNA凸出区域的OppA mRNA和tRNAIle比较了多胺对大肠杆菌OppA合成和大鼠肝脏Ile-tRNA形成的影响。结果表明，凸出区域参与多胺刺激 OppA 合成和 Ile-tRNA 形成。圆二色性证实了亚精胺对双链 RNA 凸出区域的选择性结构变化。
Polyamines play important roles in cell growth mainly through their interaction with RNA. We have previously reported that polyamines stimulate the synthesis of oligopeptide-binding protein OppA in Escherichia coli and the formation of Ile-tRNA in rat liver (Igarashi, K., and Kashiwagi, K. (2000) Biochem. Biophys. Res. Commun. 271, 559-564). These effects involve an interaction of polyamines with the bulged-out region of double-stranded RNA in the initiation region of OppA mRNA and in the acceptor stem of rat liver tRNAIle. In this study, the effects of polyamines on E. coli OppA synthesis and rat liver Ile-tRNA formation were compared using OppA mRNA and tRNAIle with or without the bulged-out region of double-stranded RNA. The results indicate that the bulged-out region is involved in polyamine stimulation of OppA synthesis and Ile-tRNA formation. A selective structural change by spermidine in the bulged-out region of double-stranded RNA was confirmed by circular dichroism.