Hyaluronic Acid Catechol: A Biopolymer Exhibiting a pH-Dependent Adhesive or Cohesive Property for Human Neural Stem Cell Engineering
Hyaluronic Acid Catechol: A Biopolymer Exhibiting a pH-Dependent Adhesive or Cohesive Property for Human Neural Stem Cell Engineering
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DOI：
10.1002/adfm.201202365
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发表时间：
2013-04-12
影响因子：
19
通讯作者：
Lee, Haeshin
中科院分区：
文献类型：
--
作者：
Hong, Seonki;Yang, Kisuk;Lee, Haeshin
大自然已经开发出了能够有效地控制其粘性和内聚性的综合材料;这些材料的化学性质在进化过程中得到了优化。例如，在海洋贻贝的粘附蛋白中发现的儿茶酚部分调节其在粘附和内聚之间的性质，迅速适应环境条件。然而，在合成材料化学中，引入的化学部分通常是单官能的，或者是粘合剂或内聚剂;通常，与天然材料相比，这是无效的。在本文中，证明了透明质酸-儿茶酚（HA-儿茶酚）缀合物可以表现出使材料表面官能化的粘附性，或构建3D水凝胶的内聚性。到目前为止，儿茶酚共轭聚合物已显示出在这两种功能之一中是有用的。证明了聚合物在干细胞工程中的有用性。可以利用HA-邻苯二酚的粘附特性制备用于神经干细胞培养的平台，并且利用HA缀合物的粘附特性制造水凝胶以包封神经干细胞。此外，HA-儿茶酚水凝胶是高度神经干细胞相容的，与现有的基于HA水凝胶的方法相比显示出更好的活力。
Nature has developed materials that are integrated and effective at controlling their properties of adhesiveness and cohesiveness; the chemistry of these materials has been optimized during evolution. For example, a catechol moiety found in the adhesive proteins of marine mussels regulates its properties between adhesion and cohesion, rapidly adapting to environmental conditions. However, in synthetic materials chemistry, introduced chemical moieties are usually monofunctional, either being adhesive or cohesive; typically, this is not effective compared to natural materials. Herein, it is demonstrated that hyaluronic acid-catechol (HA-catechol) conjugates can exhibit either adhesiveness, functionalizing the surface of materials, or cohesiveness, building 3D hydrogels. Up to now, catechol-conjugated polymers have shown to be useful in one of these two functions. The usefulness of the polymer in stem cell engineering is demonstrated. A platform for neural stem cell culture may be prepared, utilizing the adhesive property of HA-catechol, and hydrogels are fabricated to encapsulate the neural stem cells, utilizing the cohesive property of the HA conjugate. Moreover, the HA-catechol hydrogels are highly neural stem cell compatible, showing better viability compared to existing methods based on HA hydrogels.