High-Throughput Plasma Lipidomics: Detailed Mapping of the Associations with Cardiometabolic Risk Factors
High-Throughput Plasma Lipidomics: Detailed Mapping of the Associations with Cardiometabolic Risk Factors
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DOI：
10.1016/j.chembiol.2018.10.008
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发表时间：
2019-01-17
影响因子：
8.6
通讯作者：
Meikle, Peter J.
中科院分区：
文献类型：
--
作者：
Huynh, Kevin;Barlow, Christopher K.;Meikle, Peter J.
液相色谱-质谱联用（LC-MS）的高通量靶向脂质分析已被广泛用于鉴定血浆脂质种类与疾病状态之间的关联。用于表征较大临床群组的此类方法通常无法区分通常报告为脂肪酸碳和双键之和的甘油磷脂的异构形式。在这里，我们报告了一个色谱梯度加上人血浆脂质体的详细表征，以提供更好的分辨率和识别636脂质物种，包括以前未报道的物种，在15分钟的分析。我们已经利用这种方法在澳大利亚糖尿病，肥胖和生活方式研究的一个子集，并有详细的血脂种类与人体测量和血糖措施。这些结果突出了高通量脂质组学的重要性和力量，再加上脂质组的详细表征，以更好地了解人群中的脂质生物学。
High-throughput targeted lipid profiling with liquid chromatography-mass spectrometry (LC-MS) has been used extensively to identify associations between plasma lipid species and disease states. Such methods, used to characterize larger clinical cohorts, often suffer from an inability to differentiate isomeric forms of glycerophospholipids that are typically reported as the sum fatty acid carbons and double bonds. Here we report a chromatography gradient coupled with a detailed characterization of the human plasma lipidome to provide improved resolution and identification of 636 lipid species, including previously unreported species, in a 15-min analysis. We have utilized this method on a subset of the Australian Diabetes, Obesity, and Lifestyle Study and have detailed associations of plasma lipid species with anthropometric and blood glucose measures. These results highlight the importance and power of high-throughput lipidomics coupled with a detailed characterization of the lipidome to better understand lipid biology in a population setting.