Changes of Lysosomal Hydrolase Activity in the Anterior Pituitary of Hens during Induced Molting
Changes of Lysosomal Hydrolase Activity in the Anterior Pituitary of Hens during Induced Molting
复制标题
诱导蜕皮过程中母鸡垂体前叶溶酶体水解酶活性的变化
DOI：
10.2141/jpsa.39.22
复制
发表时间：
2002
影响因子：
1.5
通讯作者：
Y. Yoshimura
中科院分区：
文献类型：
--
作者：
V. S. Chowdhury;Y. Yoshimura
本研究的目的是确定在母鸡垂体诱导蜕皮过程中溶酶体水解酶活性是否发生变化。采用停饲法对来客那白龄蛋鸡进行诱导换羽试验。停止产蛋后第4 d开始逐步饲喂。分别于预处理、饥饿后3 d和5 d、停止产蛋后3 d和10 d（恢复采食后6 d）、恢复产蛋后第1天和第7 d采集母鸡垂体前叶。用酶组织化学法检测酸性磷酸酶（AcPase）活性。切片然后在光学显微镜下与图像分析计算机系统检查。各组母鸡垂体前叶头叶和尾叶细胞质中均有AcPase活性。停蛋后3 d组双叶阳性面积较预处理组显著增加。上述结果表明，停止下蛋几天后，鸡垂体前叶溶酶体酶活性有所增强，这可能是在这一阶段消化无用激素颗粒的原因。
The goal of this study was to determine whether lysosomal hydrolase activity changes during induced molting in the hen pituitary. White Leghorn aged laying hens were subjected to induced molting by feed withdrawal. Gradual feeding was started on 4th d of egg-laying cessation. The anterior pituitaries were collected from hens of pretreatment, 3 d and 5 d after starvation, 3 d and 10 d after cessation of egg-laying (6 d after resumption of feeding), on day of and 7 d after resumption of egg-laying. They were processed for the detection of acid phosphatase (AcPase) activity by enzyme histochemistry. Sections were then examined under a light microscope with an image analysis computer system. The AcPase activity was observed in the cellular cytoplasm in both cephalic and caudal lobes of the anterior pituitary in all groups of hens. In the both lobes the positive area increased significantly in the group of 3 d after cessation of egg-laying when compared with pretreatment group. These results suggest that lysosomal enzyme activity in the anterior pituitary of chicken was enhanced a few days after cessation of laying, which may be responsible for the digestion of unusable hormonal granules at this phase.