Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway.
Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway.
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DOI：
10.1016/j.neuron.2014.02.041
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发表时间：
2014-04-16
期刊：
影响因子：
16.2
通讯作者：
Khakh BS
中科院分区：
文献类型：
--
作者：
Haustein MD;Kracun S;Lu XH;Shih T;Jackson-Weaver O;Tong X;Xu J;Yang XW;O'Dell TJ;Marvin JS;Ellisman MH;Bushong EA;Looger LL;Khakh BS
成熟神经元回路中星形胶质细胞 Ca2+ 信号传导的时空活动仍不清楚。我们使用基因编码的 Ca2+ 和谷氨酸指示剂以及神经递质释放的药物遗传学和电控制来探索海马苔藓纤维通路中的星形胶质细胞活性。我们的数据显示星形胶质细胞分支和区域中存在大量局部自发 Ca2+ 信号，但这些信号不是由神经元活动或谷氨酸驱动的。此外，诱发的星形胶质细胞 Ca2+ 信号传导随苔藓纤维动作电位的数量呈线性变化。在这些设置下，星形胶质细胞的反应是全局性的，受到神经递质清除的抑制，并由谷氨酸和 GABA 介导。因此，星形胶质细胞在完全发育的苔藓纤维通路中的参与是缓慢的和领域性的，这与经常提出的微电路内星形胶质细胞的参与相反。我们表明，星形胶质细胞 Ca2+ 信号传导在功能上隔离大量神经纤维，并且这些瞬变不适合响应或调节苔藓纤维通路中的单个突触。
The spatiotemporal activities of astrocyte Ca2+ signaling in mature neuronal circuits remain unclear. We used genetically encoded Ca2+ and glutamate indicators as well as pharmacogenetic and electrical control of neurotransmitter release to explore astrocyte activity in the hippocampal mossy fiber pathway. Our data revealed numerous localised spontaneous Ca2+ signals in astrocyte branches and territories, but these were not driven by neuronal activity or glutamate. Moreover, evoked astrocyte Ca2+ signaling changed linearly with the number of mossy fiber action potentials. Under these settings astrocyte responses were global, suppressed by neurotransmitter clearance and mediated by glutamate and GABA. Thus, astrocyte engagement in the fully developed mossy fiber pathway was slow and territorial, contrary to that frequently proposed for astrocytes within microcircuits. We show that astrocyte Ca2+ signaling functionally segregates large volumes of neuropil and that these transients are not suited for responding to, or regulating, single synapses in the mossy fiber pathway.
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DOI：
10.1523/jneurosci.0127-11.2011
发表时间：
2011-06-22
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Reeves AM;Shigetomi E;Khakh BS
通讯作者：
Khakh BS
影响因子：
5.3
作者：
Rollenhagen, Astrid;Saetzler, Kurt;Luebke, Joachim H. R.
通讯作者：
Luebke, Joachim H. R.
影响因子：
5.3
作者：
Huang, YHH;Sinha, SR;Bergles, DE
通讯作者：
Bergles, DE
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
DOI：
10.1098/rspb.1967.0047
发表时间：
1967-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KUFFLER, SW
通讯作者：
KUFFLER, SW