Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
复制标题
不同缺氧复氧时间对心肌细胞自噬的影响。
DOI：
10.1590/s0102-865020180030000004
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发表时间：
2018-03-01
期刊：
影响因子：
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通讯作者：
Guo, Tao
中科院分区：
文献类型：
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作者：
Hu, Zhao;Cai, Hong-Yan;Guo, Tao
目的：方法：将大鼠心肌细胞随机分为正常对照组（A组）、缺氧组（B组）、2 h复氧组（C组）、12 h复氧组（D组）和24 h复氧组（E组）。结果：HR模型组心肌细胞存活率明显低于A组（P
Purpose: To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2).Methods: Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay.Results: Cell viabilities in HR model groups were significantly lower than those of group A (P