Effect of thromboxane synthetase inhibition on canine autogenous vein grafts.
Effect of thromboxane synthetase inhibition on canine autogenous vein grafts.
复制标题
血栓素合成酶抑制对犬自体静脉移植物的影响。
DOI：
10.1016/0022-4804(86)90190-3
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Cronenwett,JL
中科院分区：
文献类型：
--
作者：
Endean,ED;Boorstein,JM;Hees,PL;Cronenwett,JL
本研究观察了口服血栓素（TXA 2）合成酶抑制剂（TSI）对自体静脉移植物的通畅性、TXA 2生成和血小板聚集的影响。10只犬在手术前24小时开始接受TSI（U-63557 A）10 mg/kg po q8 hr，持续6周，而15只对照犬未接受治疗。收获一条颈静脉并在37°C盐水中储存1小时以诱导轻度内皮损伤（储存）。然后将正常和储存的颈静脉移植物（8 cm）植入对侧股动脉，同时将相同静脉的3 cm段植入颈动脉。在1、2、4和6周时，通过动脉造影术确定的5 Fr远端动脉狭窄和通畅性限制了股动脉移植物血流。放免法测定移植静脉内皮细胞表面血栓素B_2（TXB_2）的含量，并于颈动脉移植前24小时静脉注射~（111）In标记的血小板，以测定移植静脉内皮细胞表面血栓素B_2的含量。TSI治疗使早期（1周）股静脉移植物通畅率从63%提高到89%（P< 0.05），这一趋势持续6周。温盐水储存使1周移植物通畅率从83%降低到63%（P< 0.05），该差异随时间而减小。TSI治疗导致TXB 2的产生明显减少，但与颈静脉移植物中111 In标记的血小板沉积减少无关。温盐水储存增加了移植物血小板沉积，主要是在动脉粥样硬化。TSI治疗可改善内皮损伤短暂期的早期移植静脉通畅。
This study examined the effect of an orally active thromboxane (TXA2) synthetase inhibitor (TSI) on the patency, TXA2production, and platelet accumulation of reversed autogenous vein grafts. Ten dogs received TSI (U-63557A) 10 mg/kg po q8 hr for 6 weeks, beginning 24 hr prior to surgery, while 15 control dogs were untreated. One jugular vein was harvested and stored in 37°C saline for 1 hr to induce mild endothelial injury (stored). Normal and stored jugular vein grafts (8 cm) were then implanted in opposite femoral arteries while 3-cm segments of the same veins were implanted in the carotid arteries. Femoral graft flow was restricted with a 5 Fr distal arterial stenosis and patency determined by arteriography at 1, 2, 4, and 6 weeks. Vein graft endothelial surface TXB2production was measured by RIA at graft implantation and in carotid grafts harvested at 1 week.111In-labeled platelets were given iv 24 hr prior to carotid graft harvest to determine graft-platelet deposition. TSI treatment improved early (1 week) femoral vein graft patency from 63 to 89% (P< 0.05), a trend that persisted for 6 weeks. Warm saline storage reduced 1-week graft patency from 83 to 63% (P< 0.05), a difference that decreased with time. TSI treatment resulted in a marked decrease in TXB2production, but was not associated with decreased111In-labeled platelet deposition in carotid vein grafts. Warm saline storage increased graft-platelet deposition which was predominant at the arterial anastomoses. TSI treatment may improve early vein graft patency during the transient period of endothelial injury.