Development of a 5′ nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus
Development of a 5′ nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus
复制标题
DOI：
10.1128/jcm.41.9.4438-4441.2003
复制
发表时间：
2003-09-01
影响因子：
9.4
通讯作者：
Koga, T
中科院分区：
文献类型：
--
作者：
Yoshida, A;Suzuki, N;Koga, T
建立了5‘核酸酶TaqMan聚合酶链式反应方法，对主要致龋菌变形链球菌和远缘链球菌进行了定量检测。用该方法测定了细菌的绝对数量和相对数量。这项检测将有助于定量口腔标本中的这些微生物和分析生物膜的形成。
A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.