Mixer-settler counter-current chromatography with a barricaded spiral disk assembly with glass beads
Mixer-settler counter-current chromatography with a barricaded spiral disk assembly with glass beads
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DOI：
10.1016/j.chroma.2006.11.078
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发表时间：
2007-06-01
影响因子：
4.1
通讯作者：
Li, Ting
中科院分区：
文献类型：
--
作者：
Ito, Yoichiro;Qi, Lin;Li, Ting
设计了一种新型螺旋盘，在螺旋通道中间每隔6毫米放置障碍物，将通道分成多个部分。玻璃珠被放置在每一个其他部分，使行星运动产生重复的混合和沉淀的聚合物相系统。研究了该混合-沉淀螺旋盘组合在聚合物相体系中溶菌酶和肌红蛋白分离的性能。在转速高达1200rpm的高转速下，在螺旋盘上安装每侧开口为1.2 ~ 0.4 mm的障碍物，获得了最好的结果。(C) 2006 Elsevier B.V.版权所有
A novel spiral disk is designed by placing barricades at 6 mm intervals in the middle of the spiral channel to divide the channel into multiple sections. Glass beads are placed in every other section so that the planetary motion produces repetitive mixing and settling of polymer phase systems. Performance of this mixer-settler spiral disk assembly was examined for separation of lysozyme and myoglobin with a polymer phase system. The best results were obtained with a spiral disk equipped with barricades with openings ranging from 1.2 to 0.4 mm on each side at a high revolution speed up to 1200 rpm. (C) 2006 Elsevier B.V. All rights reserved.