LOCALIZATION OF A CIS-ACTING ELEMENT RESPONSIBLE FOR THE DEVELOPMENTALLY REGULATED AMPLIFICATION OF DROSOPHILA CHORION GENES
LOCALIZATION OF A CIS-ACTING ELEMENT RESPONSIBLE FOR THE DEVELOPMENTALLY REGULATED AMPLIFICATION OF DROSOPHILA CHORION GENES
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DOI：
10.1016/0092-8674(84)90525-7
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
SPRADLING, AC
中科院分区：
文献类型：
--
作者：
DECICCO, DV;SPRADLING, AC
在卵子发生后期，果蝇绒毛膜基因和侧翼DNA序列的两个簇在卵泡细胞中进行特异性扩增。使用P-元件介导的转化构建系，其中来自染色体III上66 D处的绒毛膜基因簇的DNA片段已插入新的染色体位置。只有转座子，含有一个特定的3.6 kb的基因组片段来自集群进行扩增卵子发生过程中，发生明显的正常组织和时间特异性。相邻的非绒毛膜序列也进行了扩增。然而，转座子差异复制的能力受到位置效应的影响。这些研究提供了证据，存在一个特定的，β-作用元件控制chollon基因扩增，其中包括一个不成比例的DNA复制的起点。用3.6 kb片段的亚片段诱导扩增的尝试是不成功的，这表明扩增可能需要该片段的大部分。
Late in oogenesis two clusters of Drosophila chorion genes and flanking DNA sequences undergo specific amplification in ovarian follicle cells. Lines were constructed using P-element-mediated transformation in which DNA segments derived from the chorion gene cluster at 66D on chromosome Ill had been inserted at new chromosomal locations. Only transposons that contained a specific 3.6 kb genomic segment derived from the cluster underwent amplification during oogenesis, which occurred with apparently normal tissue and temporal specificity. Adjacent nonchorion sequences also underwent amplification. However, the ability of a transposon to replicate differentially was subject to position effect. These studies provide evidence for the existence of a specific, &-acting element controlling chorlon gene amplification, which includes an origin for disproportionate DNA replication. Attempts to induce amplification with subfragments of the 3.6 kb segment were unsuccessful, suggesting that much of this fragment may be required for amplification.