Morphology and colonization preference of arbuscular mycorrhizal fungi in Clethra barbinervis, Cucumis sativus, and Lycopersicon esculentum
Morphology and colonization preference of arbuscular mycorrhizal fungi in Clethra barbinervis, Cucumis sativus, and Lycopersicon esculentum
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DOI：
10.1007/s10267-003-0176-2
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发表时间：
2004-06-01
期刊：
影响因子：
1.4
通讯作者：
Hyakumachi, Mitsuro
中科院分区：
文献类型：
--
作者：
Kubota, Mayumi;Hyakumachi, Mitsuro
在雪松、柏树、落叶松、红松、竹草和意大利黑麦草6个不同植被样地的土壤中，分别培养细穗草（Clethra barbinervis）、黄瓜（Cucumis sativus）和番茄（Lycopersicon esculentum），通过显微镜观察和PCR检测对丛枝菌根（arbuscular mycorrhizal， AM）真菌的形态和定植偏好进行了研究。C. barbinervis在整个站点中始终形成巴黎型AM。苜蓿同时形成Arum型AM和paris型AM， Arum型AM发生率较高。小茴香也形成了Arum- AM和Paris-type AM，但Paris-type AM发生率较高。同一种植物内AM多样性在不同立地之间存在差异。不同地点土壤中AM孢子中检测到的AM多样性与试验植物中AM真菌的多样性并不一致。检测到的科是不同的，这取决于在同一土壤中生长的试验植物。所有试验点的植物根部均检测到属于Glomaceae的AM真菌。几乎所有的科都是从巴比纳藤和石竹根中检测到的。另一方面，在sativus根中只检测到两个或三个AM真菌科（Archaeosporaceae和/或Paraglomaceae和Glomaceae），而没有检测到两个AM真菌科（Acaulosporaceae和Gigasporaceae），说明AM真菌在试验植物中对sativus有很强的定殖偏好。本研究表明寄主植物种类对AM真菌在根系中的定殖偏好有强烈的影响。
Clethra barbinervis (Ericales), Cucumis sativus, and Lycopersicon esculentum were grown in soils collected from six different vegetation sites (cedar, cypress, larch, red pine, bamboo grass, and Italian ryegrass), and morphology and colonization preference of arbuscular mycorrhizal (AM) fungi were investigated by microscopic observation and PCR detection. C. barbinervis consistently formed Paris-type AM throughout the sites. C. sativus formed both Arum- and Paris-type AM with high occurrence of Arum-type AM. L. esculentum also formed both Arum- and Paris-type AM but with high occurrence of Paris-type AM. AM diversity within the same plant species was different among the sites. Detected AM diversity from AM spores in different site soils did not consistently reflect AM fungal diversity seen in test plants. Detected families were different, depending on test plants grown even in the same soil. AM fungi belonging to Glomaceae were consistently detected from roots of all test plants throughout the sites. Almost all the families were detected from roots of C. barbinervis and L. esculentum. On the other hand, only two or three families of AM fungi (Archaeosporaceae and/or Paraglomaceae and Glomaceae) but not two other families (Acaulosporaceae and Gigasporaceae) were detected from roots of C. sativus, indicating strong colonization preference of AM fungi to C. sativus among test plants. This study demonstrated that host plant species strongly influenced the colonization preference of AM fungi in the roots.