Human and Mouse Macrophages Collaborate with Neutrophils To Kill Larval Strongyloides stercoralis
Human and Mouse Macrophages Collaborate with Neutrophils To Kill Larval Strongyloides stercoralis
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DOI：
10.1128/iai.00625-13
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发表时间：
2013-09-01
影响因子：
3.1
通讯作者：
Abraham, David
中科院分区：
文献类型：
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作者：
Bonne-Annee, Sandra;Kerepesi, Laura A.;Abraham, David
巨噬细胞是一种在T(H)1和T(H)2介导的反应中活跃的多功能细胞。在这项研究中，我们证明了人和小鼠的巨噬细胞与中性粒细胞和补体合作，在体外杀死了寄生虫类圆线虫。感染蠕虫的小鼠可在腹膜腔内诱导交替激活的巨噬细胞(AAM Phi)。这些细胞在体内杀死蠕虫，并在体外杀死过程中与中性粒细胞和补体合作。在体外产生的AAM Phi杀死幼虫的速度比幼虫巨噬细胞更快，后者在较长时间后杀死幼虫。相比之下，经典激活的巨噬细胞在体外或体内都不能杀死幼虫。这项研究将巨噬细胞添加到免疫成分中，这些免疫成分具有消除寄生蠕虫的功能，并展示了AAM Phi控制大型细胞外寄生虫的一种新功能。
Macrophages are multifunctional cells that are active in T(H)1- and T(H)2-mediated responses. In this study, we demonstrate that human and mouse macrophages collaborate with neutrophils and complement to kill the parasite Strongyloides stercoralis in vitro. Infection of mice with worms resulted in the induction of alternatively activated macrophages (AAM phi) within the peritoneal cavity. These cells killed the worms in vivo and collaborated with neutrophils and complement during the in vitro killing process. AAM phi generated in vitro killed larvae more rapidly than naive macrophages, which killed larvae after a longer time period. In contrast, classically activated macrophages were unable to kill larvae either in vitro or in vivo. This study adds macrophages to the armamentarium of immune components that function in elimination of parasitic helminths and demonstrate a novel function by which AAM phi control large extracellular parasites.