Biotransformation and Metabolic Profile of Limonin in Rat Liver Microsomes, Bile, and Urine by High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry
Biotransformation and Metabolic Profile of Limonin in Rat Liver Microsomes, Bile, and Urine by High-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry
复制标题
采用高效液相色谱结合四极杆飞行时间质谱法测定大鼠肝微粒体、胆汁和尿液中柠檬苦素的生物转化和代谢特征
DOI：
10.1021/acs.jafc.8b02057
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发表时间：
2018-10-10
影响因子：
6.1
通讯作者：
Ju, Wenzheng
中科院分区：
文献类型：
--
作者：
Liu, Shijia;Dai, Guoliang;Ju, Wenzheng
柠檬苦素是柑橘种子中的一种三萜类化合物，具有重要的生物活性。然而，柠檬苦素的代谢特征尚未完全了解。为了阐明柠檬苦素的体内、外代谢，本文研究了柠檬苦素在大鼠肝微粒体、尿液和胆汁中的代谢产物。采用高效液相色谱/四极杆飞行时间质谱法进行鉴别。其中代谢产物M1和M3的结构经化学合成和核磁共振谱分析得到证实。结果表明，还原和水解是柠檬苦素体内外代谢的两条主要途径。本研究结果对了解柠檬苦素的代谢过程具有重要意义。
Limonin is a triterpenoid in citrus seeds, which has significant biological activities. However, the metabolic profile of limonin has not been fully understood. To expound its metabolism in vivo and in vitro, the metabolites of limonin was studied by rat liver microsomes, urine, and bile. High-performance liquid chromatography/quadrupole time-of-flight mass spectrometry was used for identification. Among the metabolites, the structures of M1 and M3 were confirmed by chemical synthesis and nuclear magnetic resonance spectra analysis. Our results indicated that reduction and hydrolysis were the two major pathways during limonin metabolism in vivo and in vitro. The results from this work are valuable and important for understanding the metabolic process of limonin.