On the ecosystemic network of saliva in healthy young adults.
On the ecosystemic network of saliva in healthy young adults.
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DOI：
10.1038/ismej.2016.199
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Keijser BJ
中科院分区：
文献类型：
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作者：
Zaura E;Brandt BW;Prodan A;Teixeira de Mattos MJ;Imangaliyev S;Kool J;Buijs MJ;Jagers FL;Hennequin-Hoenderdos NL;Slot DE;Nicu EA;Lagerweij MD;Janus MM;Fernandez-Gutierrez MM;Levin E;Krom BP;Brand HS;Veerman EC;Kleerebezem M;Loos BG;van der Weijden GA;Crielaard W;Keijser BJ
据信，生态失调状态是口腔疾病发病的关键因素。虽然口腔疾病已经研究了几十年，但我们对口腔健康的理解，健康口腔生态系统的边界和生态向生态失调的转变仍然有限。在这里，我们提出了唾液生态系统的生态生物学异质性和唾液微生物组，唾液代谢组和宿主相关的生化唾液参数之间的关系，在268名健康成人过夜禁食。观察到微生物组和代谢组的性别特异性差异，并与唾液pH值和膳食蛋白质摄入量相关。我们的分析将个体分为五个微生物组和四个基于代谢组的聚类，这些聚类与唾液的生化参数显著相关。低唾液pH值和高溶菌酶活性与高比例的链球菌E2型和增加膜脂降解产物。高唾液pH值的样品显示几丁质酶活性增加，韦荣氏球菌和普雷沃氏菌物种的丰度较高，氨基酸发酵产物的水平较高，这表明蛋白水解适应。对蛋白水解或糖分解生态型的过度特化可能表明向生态失调状态的转变。它们的预后价值和这些生态类型与疾病风险增加相关的程度仍有待确定。
A dysbiotic state is believed to be a key factor in the onset of oral disease. Although oral diseases have been studied for decades, our understanding of oral health, the boundaries of a healthy oral ecosystem and ecological shift toward dysbiosis is still limited. Here, we present the ecobiological heterogeneity of the salivary ecosystem and relations between the salivary microbiome, salivary metabolome and host-related biochemical salivary parameters in 268 healthy adults after overnight fasting. Gender-specific differences in the microbiome and metabolome were observed and were associated with salivary pH and dietary protein intake. Our analysis grouped the individuals into five microbiome and four metabolome-based clusters that significantly related to biochemical parameters of saliva. Low salivary pH and high lysozyme activity were associated with high proportions of streptococcal phylotypes and increased membrane-lipid degradation products. Samples with high salivary pH displayed increased chitinase activity, higher abundance of Veillonella and Prevotella species and higher levels of amino acid fermentation products, suggesting proteolytic adaptation. An over-specialization toward either a proteolytic or a saccharolytic ecotype may indicate a shift toward a dysbiotic state. Their prognostic value and the degree to which these ecotypes are related to increased disease risk remains to be determined.