Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch
Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch
复制标题
DOI：
10.1007/s00345-015-1509-4
复制
发表时间：
2015-11-01
影响因子：
3.4
通讯作者：
Wang, Kun-Jie
中科院分区：
文献类型：
--
作者：
Luo, De-Yi;Wazir, Romel;Wang, Kun-Jie
本研究的目的是描述和测试一种基于改进的BOSE生物动力学系统的拉伸模式，以在膀胱周期中产生最佳的生理拉伸。将人膀胱平滑肌细胞（HBSMCs）接种于硅胶膜上，在改良的BOSE BioDynamic生物反应器上模拟膀胱周期，采用自行设计的软件，模拟膀胱周期，观察生理性牵张幅度对HBSMCs增殖和收缩能力的影响。采用免疫荧光和激光共聚焦显微镜观察细胞形态学变化。分别用BrdU掺入法和Cell Counting Kit-8测定细胞增殖和细胞活力。使用胶原凝胶收缩测定法测定细胞的收缩性。RT-PCR检测细胞表型和收缩力，结果显示，在改良的生物反应器中，HBSMCs在不同的延伸率下均呈梭形和定向生长。牵张诱导的增殖和存活依赖于牵张的大小，牵张也以大小依赖的方式调节收缩性和收缩标记物。结果还表明，机械牵张可以促进体外HBSMCs的形态、增殖和收缩的幅度依赖性调节。
The purpose of this study was to describe and test a kind of stretch pattern which is based on modified BOSE BioDynamic system to produce optimum physiological stretch during bladder cycle. Moreover, we aimed to emphasize the effects of physiological stretch's amplitude upon proliferation and contractility of human bladder smooth muscle cells (HBSMCs).HBSMCs were seeded onto silicone membrane and subjected to stretch simulating bladder cycle at the range of stretches and time according to customized software on modified BOSE BioDynamic bioreactor. Morphological changes were assessed using immunofluorescence and confocal laser scanning microscope. Cell proliferation and cell viability were determined by BrdU incorporation assay and Cell Counting Kit-8, respectively. Contractility of the cells was determined using collagen gel contraction assay. RT-PCR was used to assess phenotypic and contractility markers.HBSMCs were found to show morphologically spindle-shaped and orientation at various elongations in the modified bioreactor. Stretch-induced proliferation and viability depended on the magnitude of stretch, and stretches also regulate contractility and contraction markers in a magnitude-dependent manner.We described and tested a kind of stretch pattern which delivers physiological stretch implemented during bladder cycle. The findings also showed that mechanical stretch can promote magnitude-dependent morphological, proliferative and contractile modulation of HBSMCs in vitro.