Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media
Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media
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DOI：
10.3390/jcm8111853
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发表时间：
2019-11-01
影响因子：
3.9
通讯作者：
Kim, Jae-Seok
中科院分区：
文献类型：
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作者：
Lade, Harshad;Park, Joon Hyun;Kim, Jae-Seok
金黄色葡萄球菌（金黄色葡萄球菌）引起持续性生物膜相关感染。金黄色葡萄球菌的生物膜形成受培养条件的影响，并与某些基因型特征有关。在这里，我们发现在培养基中添加葡萄糖和氯化钠（NaCl），这是体外生物膜研究中的一种常见做法，会影响40种金黄色葡萄球菌临床分离株（耐甲氧西林和甲氧西林敏感的金黄色葡萄球菌）的生物膜形成，并导致生物膜定量的变化。甲氧西林耐药菌株在胰蛋白酶豆汤（TSB）中形成的生物膜比甲氧西林敏感菌株更坚固。然而，在TSB中添加葡萄糖极大地促进和稳定了所有菌株的生物膜形成，而添加NaCl在这方面的效率较低，导致生物膜测量结果的显著变化。此外，我们观察到ST239-SCCmec（葡萄球菌盒染色体mec） III型谱系在添加葡萄糖和NaCl的TSB中形成了很强的生物膜。生物膜的形成与辅助基因调节因子（agr）状态之间的联系，通过三角毒素的产生来评估，与甘露醇发酵没有发现。我们的研究结果表明，在TSB中添加1.0%的葡萄糖可以促进体外金黄色葡萄球菌生物膜的生成和可重复的定量，而在TSB中添加NaCl会导致生物膜形成的测量结果发生重大变化。
Staphylococcus aureus (S. aureus) causes persistent biofilm-related infections. Biofilm formation by S. aureus is affected by the culture conditions and is associated with certain genotypic characteristics. Here, we show that glucose and sodium chloride (NaCl) supplementation of culture media, a common practice in studies of biofilms in vitro, influences both biofilm formation by 40 S. aureus clinical isolates (methicillin-resistant and methicillin-sensitive S. aureus) and causes variations in biofilm quantification. Methicillin-resistant strains formed more robust biofilms than methicillin-sensitive strains in tryptic soy broth (TSB). However, glucose supplementation in TSB greatly promoted and stabilized biofilm formation of all strains, while additional NaCl was less efficient in this respect and resulted in significant variation in biofilm measurements. In addition, we observed that the ST239-SCCmec (Staphylococcal Cassette Chromosome mec) type III lineage formed strong biofilms in TSB supplemented with glucose and NaCl. Links between biofilm formation and accessory gene regulator (agr) status, as assessed by delta-toxin production, and with mannitol fermentation were not found. Our results show that TSB supplemented with 1.0% glucose supports robust biofilm production and reproducible quantification of S. aureus biofilm formation in vitro, whereas additional NaCl results in major variations in measurements of biofilm formation.