Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs
Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs
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DOI：
10.1152/ajplung.00056.2013
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发表时间：
2013-08-01
影响因子：
4.9
通讯作者：
Williams, Katherine E.
中科院分区：
文献类型：
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作者：
Bartlett, Jennifer A.;Albertolle, Matthew E.;Williams, Katherine E.
气道粘膜和肺泡表面形成肺与外部环境之间的动态界面。排列在这些屏障上的上皮细胞精心制作了一层薄薄的液体层，其中含有有助于宿主防御和其他功能的分泌肽和蛋白质。本研究的目的是开发和应用方法来定义猪肺衬里液的蛋白质组，部分是通过利用猪Ensembl基因，转录本和蛋白质模型预测数据库中的丰富信息。我们开发了一种优化的工作流程，用于检测猪支气管肺泡灌洗液（BAL）和乙酰甲胆碱诱导的气管分泌物[气道表面液体（ASL）]中的分泌蛋白。我们在BAL和ASL中分别检测到674和3，858种独特的猪特异性蛋白。该蛋白质组由代表多种分子类别和生物过程的蛋白质组成，包括宿主防御、分子运输、细胞通讯、细胞骨架和代谢功能。具体来说，我们检测到大量的分泌蛋白，已知或预测的先天性和适应性免疫，微生物杀伤，或宿主防御的其他方面的作用。在极大地扩大了已知的蛋白质组的肺内衬液在猪，这项研究提供了一个宝贵的资源，为未来的研究使用这一重要的动物模型的肺生理和疾病。
The airway mucosa and the alveolar surface form dynamic interfaces between the lung and the external environment. The epithelial cells lining these barriers elaborate a thin liquid layer containing secreted peptides and proteins that contribute to host defense and other functions. The goal of this study was to develop and apply methods to define the proteome of porcine lung lining liquid, in part, by leveraging the wealth of information in the Sus scrofa database of Ensembl gene, transcript, and protein model predictions. We developed an optimized workflow for detection of secreted proteins in porcine bronchoalveolar lavage (BAL) fluid and in methacholine-induced tracheal secretions [airway surface liquid (ASL)]. We detected 674 and 3,858 unique porcine-specific proteins in BAL and ASL, respectively. This proteome was composed of proteins representing a diverse range of molecular classes and biological processes, including host defense, molecular transport, cell communication, cytoskeletal, and metabolic functions. Specifically, we detected a significant number of secreted proteins with known or predicted roles in innate and adaptive immunity, microbial killing, or other aspects of host defense. In greatly expanding the known proteome of the lung lining fluid in the pig, this study provides a valuable resource for future studies using this important animal model of pulmonary physiology and disease.