Construction of Protein Nanowires through Cucurbit[8]uril-based Highly Specific HostGuest Interactions: An Approach to the Assembly of Functional Proteins
Construction of Protein Nanowires through Cucurbit[8]uril-based Highly Specific HostGuest Interactions: An Approach to the Assembly of Functional Proteins
复制标题
通过基于葫芦[8]脲的高度特异性主客体相互作用构建蛋白质纳米线：一种功能蛋白质组装方法
DOI：
10.1002/anie.201300692
复制
发表时间：
2013-01-01
影响因子：
16.6
通讯作者：
Liu, Junqiu
中科院分区：
文献类型：
--
作者：
Hou, Chunxi;Li, Jiaxi;Liu, Junqiu
蛋白质纳米结构不仅具有生物相容性和可生物降解的特性，而且具有较高的结构耐久性，便于回收利用，因此在仿生学领域引起了极大的兴趣。一些创造性的策略已经发展到构建基于分子识别的蛋白质纳米结构，如对称，金属离子诱导，或超分子相互作用一些蛋白质纳米结构，如人造蛋白质纳米线、[1a]蛋白质环、[1b]和蛋白质球，[1c]已经通过这些策略被创造出来，并应用于催化、组织成像、诊断和治疗。其中，主客体超分子相互作用以可逆和生物正交的方式提供了理想的驱动力。虽然利用蛋白质-配体相互作用已经成功地开发了主-客相互作用，如生物素-链亲和素，[2a， b]二氢叶酸还原酶-甲氨蝶呤，[2c]和凝集素魔豆蛋白a -甘露pyranoside，[2d]，通过小分子主-客相互作用构建功能性蛋白质纳米结构的研究很少。Cucurbit [n] uril是一系列鲁棒大环寄主（CB [n]; n= 5 - 8,10）由于CB在水介质中具有很强的客体结合能力，因此CB同源物在超分子纳米结构的设计中起着重要的作用。[3a]基于cb的主客体相互作用已经成功构建了从低聚物到三维结构的超分子结构。例如，Kim及其同事通过CB[8]介导的与亚甲基桥接萘-二吡啶六甲基乙烯客体的相互作用，开发出了分子项链。[4a] Scherman和同事们报道了一系列由强可逆的基于CB b[8]的1:1:1三联基结合基序组成的超分子水凝胶和微胶囊，通常以甲基紫紫素为第一客体，萘氧基衍生物为第二客体。[4b， c]此外，Zhang和同事还构建了由CB[8]和1-（蒽-2-）形成的超分子聚合物
Protein nanostructures are of great interest in the field of bionics, owing not only to their biocompatible and biodegradable properties, but also to their high structural durability, which facilitates recycling. Some creative strategies have been developed to construct protein nanostructures based on molecular recognition, such as symmetry, metal ion induction, or supramolecular interactions.[1] Some protein nanostructures, such as artificial protein nanowires,[1a] protein rings,[1b] and protein spheres,[1c] have been created by these strategies and applied in catalysis, tissue imaging, diagnostics, and therapeutic treatments. Among them, host–guest supramolecular interactions have provided desirable driving forces in a reversible and bioorthogonal manner. Although host–guest interactions have been successfully developed using protein–ligand interactions, such as biotin–streptavidin,[2a, b] dihydrofolate reductase–methotrexate,[2c] and lectin concanavalin A–mannopyranoside,[2d] the construction of functional protein nanostructures by small molecule host–guest interactions has rarely been explored.Cucurbit [n] uril is a series of robust macrocyclic hosts (CB [n]; n= 5–8, 10).[3] Owing to the great guest-binding ability of CB in aqueous media, CB homologues play a major role in the design of supramolecular nanostructures.[3a] Supramolecular structures ranging from oligomers to 3D structures have been successfully constructed by CB-based host–guest interactions. For example, Kim and co-workers have developed molecular necklaces through CB [8]-mediated interactions with a methylene-bridged naphthalene–dipyridyliumylethylene guest.[4a] Scherman and co-workers have reported a series of supramolecular hydrogels and microcapsules comprised of a strong, reversible CB [8]-based 1: 1: 1 ternary binding motif, typically with methyl viologen as the first guest and naphthoxy derivatives as the second guest.[4b, c] Furthermore, Zhang and co-workers have constructed supramolecular polymers formed by CB [8] and 1-(anthracen-2-