FINE STRUCTURE OF KINETOCHORE OF A MAMMALIAN CELL IN VITRO
FINE STRUCTURE OF KINETOCHORE OF A MAMMALIAN CELL IN VITRO
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DOI：
10.1007/bf00332792
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发表时间：
1966-01-01
期刊：
影响因子：
1.6
通讯作者：
STUBBLEFIELD, E
中科院分区：
文献类型：
--
作者：
BRINKLEY, BR;STUBBLEFIELD, E
用电子显微镜观察了中国仓鼠细胞的染色体，并对着丝粒的结构和组织进行了观察。动粒由一个直径200-300A的致密核心组成，周围环绕着一个较不致密的200-600A宽的区域。致密的核心由一对直径50-80A的轴向纤维组成，它们可以以共螺旋的方式盘绕在一起。轴向元件周围较不致密的区域由许多与轴向纤维成直角的微纤维组成。这些结构一起组成了一个灯刷状的细丝，它沿着每个色谱柱的表面延伸。一些切片表明，在每个染色单体上可能存在2个这样的细丝。纺锤丝伴生的动点的精细结构与无纺锤丝的动点基本相同。将这些哺乳动物细胞的动粒的结构和组织与某些两栖类卵母细胞的灯刷染色体、双翅目多线染色体以及减数分裂前期所见的联会复合体进行了比较。
The chromosomes of Chinese hamster cells were examined with the electron microscope and the following observations were made concerning the structure and organization of the kinetochore. The kinetochore consists of a dense core 200-300 A In diameter surrounded by a less dense zone 200-600 A wide. The dense core consists of a pair of axial fibrils 50-80 A in diameter which may be coiled together in a cohelical manner. The less dense zone about the axial elements is composed of a numerous microffbrils which loop out at right angles to the axial fibrils. Together the structures comprise a lampbrush-like filament which extends along the surface of each chromatld. Some sections suggested that 2 such filaments may be present on each chromatid. The fine structure of kinetochores associated with spindle filaments was essentially the same as those free of filaments. The structure and organization of the kinetochore of these mammalian cells was compared to that of lampbrush chromosomes of certain amphibian oocytes, dipteran polytene chromosome puffs, and the synaptinemal complex seen during meiotic prophase.