Organization of Transcription
Organization of Transcription
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DOI：
10.1101/cshperspect.a000729
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发表时间：
2010-09-01
影响因子：
7.2
通讯作者：
Fraser, Peter
中科院分区：
文献类型：
--
作者：
Chakalova, Lyubomira;Fraser, Peter
对转录组织的研究起源于细胞生物学。早期的研究表明，新生转录与可辨别的核结构和成分有关。光学显微镜、免疫荧光和原位杂交技术的进步帮助我们开始这项艰巨的任务，即命名无数的个体参与者和转录组分，并将它们置于背景中。随着哺乳动物基因组序列的完成，理解基因组转录的看似无限的任务变得有限，并开始了一个快速发展的新时期。在这里，我们专注于组织的转录哺乳动物借鉴信息从低等生物在必要的地方。新出现的图像是一个高度组织化的细胞核，具有适合组织特异性转录和基因表达程序的基因组特定构象。
Investigations into the organization of transcription have their origins in cell biology. Early studies characterized nascent transcription in relation to discernable nuclear structures and components. Advances in light microscopy, immunofluorescence, and in situ hybridization helped to begin the difficult task of naming the countless individual players and components of transcription and placing them in context. With the completion of mammalian genome sequences, the seemingly boundless task of understanding transcription of the genome became finite and began a new period of rapid advance. Here we focus on the organization of transcription in mammals drawing upon information from lower organisms where necessary. The emerging picture is one of a highly organized nucleus with specific conformations of the genome adapted for tissue-specific programs of transcription and gene expression.