Urocortins are present in the rat testis.
Urocortins are present in the rat testis.
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DOI：
10.1016/j.npep.2010.12.006
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发表时间：
2011-04
期刊：
影响因子：
2.9
通讯作者：
Rivier, Catherine
中科院分区：
文献类型：
--
作者：
Lee, Soon;Braden, Brian;Kang, Sang Soo;Rivier, Catherine
The synthesis and release of testosterone (T) depends both on circulating luteinizing hormone (LH) and on an array of testicular factors whose role remains incompletely understood. Corticotropin-releasing factor (CRF) had been reported in the rat testes, where it was thought to inhibit T secretion. However, the discovery that the CRF-related peptides urocortins (Ucns), of which there are currently 3 subtypes (Ucn 1, 2 and 3), cross-react with many reagents previously used to detect CRF, has cast doubt on this concept. Here we show that while CRF was readily measurable in rat hypothalami (which served as controls), signals for this peptide were barely detectable in total RNA extracted from the testes. On the other hand, microarray, RT-PCR and real-time quantitative RT-PCR (qRT-PCR) analyses all indicated strong signals for Ucn 1 in the male gonads, with weaker levels of Ucn 2 and 3 mRNA gene expression. Results obtained for Ucn 1 gene expression were corroborated by immunohistochemical detection, which appeared restricted to Leydig cells. Finally, to investigate possible changes in testicular Ucn 1 levels induced by homeostatic challenges, we measured them in rats exposed to alcohol. We observed that indeed, the intragastric injection of this drug significantly increased testicular Ucn 1, but not Ucn 2, Ucn 3, CRF, CRFR1 or CRFR2 mRNA levels. Collectively, these results provide novel information regarding the presence of CRF-like peptides in the adult male rat testis.
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影响因子：
4.8
作者：
Donaldson, CJ;Sutton, SW;Vale, WW
通讯作者：
Vale, WW
DOI：
10.1016/0167-4838(89)90226-4
发表时间：
1989-03-16
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
AUDHYA, T;HOLLANDER, CS;HUTCHINSON, B
通讯作者：
HUTCHINSON, B
影响因子：
--
作者：
LEFEBVRE, DL;ZINGG, HH
通讯作者：
ZINGG, HH
DOI：
10.1073/pnas.121165198
发表时间：
2001-06-19
影响因子：
11.1
作者：
Lewis, K;Li, C;Vale, WW
通讯作者：
Vale, WW
影响因子：
4.8
作者：
ESKELAND, NL;LUGO, DI;SCHACHTER, BS
通讯作者：
SCHACHTER, BS