Suppression of tumor growth and enhancement of immune status with high levels of dietary vitamin B6 in BALB/c mice.
Suppression of tumor growth and enhancement of immune status with high levels of dietary vitamin B6 in BALB/c mice.
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高水平饮食维生素 B6 可抑制 BALB/c 小鼠的肿瘤生长并增强免疫状态。
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Shultz Td
中科院分区：
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作者：
D. Gridley;D. Stickney;R. L. Nutter;J. Slater;Shultz Td
研究了从缺乏到大剂量水平的膳食维生素B6对单纯疱疹病毒2型转化（H238）细胞诱导的肿瘤的发展以及与细胞介导的免疫相关的体外反应的影响。雄性BALB/cByJ小鼠（n = 260），5周龄，喂养20%酪蛋白饮食含有吡哆醇（PN）在0.2，1.2的对照饮食，7.7，或74.3毫克/公斤的饮食4-11周。饮食治疗4周后，120只小鼠接受H238细胞注射;未注射H238的小鼠作为对照。在第4、8和11周，对每组动物实施安乐死，并采集血液和脾脏样品。喂食0.2 mg PN的小鼠出现轻度缺乏症状，体重增加显著低于喂食1.2、7.7和74.3 mg PN饲料的小鼠。肿瘤细胞注射后13 - 16天，喂食74.3 mg PN的小鼠的原发性肿瘤发生率最低;之后，各组间的发生率相似。在注射后7周，喂食1.2 mg PN的小鼠具有最大的原发性肿瘤体积、最高的肺转移发生率和最大数量的转移性结节/动物。总体而言，在喂食7.7和74.3 mg PN的动物中发现肿瘤体积较低（分别比喂食1.2 mg PN的动物的肿瘤体积小14%和32%）;喂食0.2 mg PN的小鼠的肿瘤体积最低。与喂食0.2或1.2 mg PN的动物相比，喂食7.7和74.3 mg PN的小鼠对植物血凝素或伴刀豆球蛋白A刺激的血液和脾脏淋巴增殖反应通常倾向于更高。然而，在所有进行性肿瘤生长的动物中观察到有丝分裂原刺激的反应性降低。肿瘤生长还导致脾肿大和胸腺萎缩增加。在1.2、7.7和74.3 mg PN饮食组中观察到肿瘤体积与肿瘤5-磷酸吡哆醛（PLP）浓度之间存在显著的负相关关系。这些数据表明，饮食中摄入大量维生素B6可能通过免疫增强或PLP对肿瘤生长的调节来抑制肿瘤的发展。
Effects of dietary vitamin B6 at levels ranging from deficiency to megadoses on the development of herpes simplex virus type 2-transformed (H238) cell-induced tumors and on in vitro responses relating to cell-mediated immunity were examined. Male BALB/cByJ mice (n = 260), 5 weeks of age, were fed 20% casein diets containing pyridoxine (PN) at 0.2, 1.2 for the control diet, 7.7, or 74.3 mg/kg diet for 4-11 weeks. After 4 weeks of dietary treatment, 120 of the mice received an injection of H238 cells; mice without H238 injection served as controls. At 4, 8, and 11 weeks, animals from each group were euthanized and blood and spleen samples obtained. Mice fed 0.2 mg PN developed mild deficiency symptoms and gained significantly less weight than those fed 1.2-, 7.7-, and 74.3-mg PN diets. Thirteen to 16 days after tumor cell injection, primary tumor incidence was lowest in mice fed 74.3 mg PN; later, incidence among groups was similar. Mice fed 1.2 mg PN had the largest primary tumor volume, the highest incidence of lung metastases, and the greatest number of metastatic nodules per animal at 7 weeks post injection. Overall, lower tumor volumes were found in animals fed 7.7 and 74.3 mg PN (14 and 32% less than the tumor volume for those fed 1.2 mg PN, respectively); mice fed 0.2 mg PN had the lowest tumor volume. Blood and spleen lymphoproliferative response to stimulation by phytohemagglutinin or concanavalin A generally tended to be higher in mice fed 7.7 and 74.3 mg PN as compared to that in animals fed either 0.2 or 1.2 mg PN. However, decreased mitogen-stimulated responsiveness was observed in all animals with progressive tumor growth. Tumor growth also resulted in splenomegaly and increased thymic atrophy. Significant negative relationships between tumor volume and tumor pyridoxal 5-phosphate (PLP) concentrations were observed for 1.2-, 7.7-, and 74.3-mg PN diet groups. These data suggest that high dietary intake of vitamin B6 may have suppressed tumor development by either immune enhancement or PLP growth regulation of this tumor.