Role of CD9 in Sperm-Egg Fusion and Its General Role in Fusion Phenomena
Role of CD9 in Sperm-Egg Fusion and Its General Role in Fusion Phenomena
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DOI：
10.1007/978-90-481-9772-9_7
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
N. Kawano;Yuichirou Harada;Keiichi Yoshida;M. Miyado;K. Miyado
中科院分区：
文献类型：
--
作者：
N. Kawano;Yuichirou Harada;Keiichi Yoshida;M. Miyado;K. Miyado
在受精过程中，两种类型的性细胞或配子--精子和卵子--以一种循序渐进的方式结合形成母细胞，母细胞能够自然发育成新的个体。值得注意的是，精子和卵子之间发生的细胞间“膜融合”是受精所必需的。精子因子通过融合进入卵子细胞质，激活信号通路，导致卵子恢复减数分裂。在哺乳动物中，精子-卵子融合部分是由两种完整的膜蛋白--精子出云(Inoue et al.2005)和卵群分化9(CD9)(Kaji等人)。2000年，Le Naour等人。2000年，Miyado et al.2000年)，这两个人所发挥的作用是关键的，但尚不清楚。最近的一项研究(Miyado等人)2000)的研究表明，含有CD9的囊泡是从野生型卵子中释放出来的，尽管CD9缺失的卵子对精卵融合具有高度的抵抗力，但这些外切体样的囊泡在体外可以诱导精子和CD9缺失的卵子之间的融合。这一结果为含有CD9的、促进融合的小泡在精子-卵子融合中的关键参与提供了令人信服的证据，并为配子融合和其他膜融合事件提供了新的见解。
In fertilization, two types of sex cells or gametes – a sperm and an egg – unite in a stepwise manner to form a mother cell, which is capable of developing naturally into a new individual. Notably, the “membrane fusion” that occurs intercellularly between a sperm and an egg is essential for fertilization. A sperm factor that is delivered into the egg cytoplasm through fusion serves to activate a signaling pathway; this leads to the resumption of meiosis in the egg. In mammals, sperm-egg fusion is partly mediated by two integral membrane proteins, sperm Izumo (Inoue et al. 2005) and egg cluster of differentiation 9 (CD9) (Kaji et al. 2000, Le Naour et al. 2000, Miyado et al. 2000), and the roles played by both are critical but yet unknown. A recent study (Miyado et al. 2000) showed that CD9-containing vesicles are released from wild-type eggs, and that these exosome-like vesicles induce fusion between sperm and CD9-null eggs in vitro, even though CD9-null eggs are highly refractory to sperm-egg fusion. This result provides compelling evidence for the crucial involvement of CD9-containing, fusion-facilitating vesicles in sperm-egg fusion and offers new insight into both gamete fusion and other membrane fusion events.