A Recombinant Rift Valley Fever Virus Glycoprotein Subunit Vaccine Confers Full Protection against Rift Valley Fever Challenge in Sheep.
A Recombinant Rift Valley Fever Virus Glycoprotein Subunit Vaccine Confers Full Protection against Rift Valley Fever Challenge in Sheep.
复制标题
重组裂谷发烧病毒糖蛋白亚基疫苗赋予绵羊中裂谷发烧挑战的完全保护。
DOI：
10.1038/srep27719
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发表时间：
2016-06-14
影响因子：
4.6
通讯作者：
Richt JA
中科院分区：
文献类型：
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作者：
Faburay B;Wilson WC;Gaudreault NN;Davis AS;Shivanna V;Bawa B;Sunwoo SY;Ma W;Drolet BS;Morozov I;McVey DS;Richt JA
裂谷热病毒（RVFV）是一种蚊媒人畜共患病原体，在非洲和阿拉伯半岛引起疾病暴发。由于在非流行地区存在感病媒介，该病毒具有很大的跨界传播潜力。目前没有完全许可的疫苗适用于流行地区以外的牲畜或人类。在这里，我们报告了基于RVFV Gn和Gc糖蛋白的重组亚单位疫苗的效力的评价。在先前的研究中，该疫苗在绵羊中引发了强烈的病毒中和抗体应答，并且与DIVA（区分自然感染和接种疫苗的动物）相容。在当前的有效性研究中，一组绵羊（n = 5）皮下接种基于糖蛋白的亚单位疫苗候选物，然后用强毒Kenya-128 B-15 RVFV毒株进行异源攻毒。该疫苗引起高病毒中和抗体滴度，并在所有接种的绵羊中赋予完全保护，如通过预防病毒血症、发热和不存在RVFV相关的组织病理学病变所证明的。我们的结论是，亚单位疫苗平台是一个很有前途的战略，在易感宿主的RVFV感染的预防和控制。
Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen causing disease outbreaks in Africa and the Arabian Peninsula. The virus has great potential for transboundary spread due to the presence of competent vectors in non-endemic areas. There is currently no fully licensed vaccine suitable for use in livestock or humans outside endemic areas. Here we report the evaluation of the efficacy of a recombinant subunit vaccine based on the RVFV Gn and Gc glycoproteins. In a previous study, the vaccine elicited strong virus neutralizing antibody responses in sheep and was DIVA (differentiating naturally infected from vaccinated animals) compatible. In the current efficacy study, a group of sheep (n = 5) was vaccinated subcutaneously with the glycoprotein-based subunit vaccine candidate and then subjected to heterologous challenge with the virulent Kenya-128B-15 RVFV strain. The vaccine elicited high virus neutralizing antibody titers and conferred complete protection in all vaccinated sheep, as evidenced by prevention of viremia, fever and absence of RVFV-associated histopathological lesions. We conclude that the subunit vaccine platform represents a promising strategy for the prevention and control of RVFV infections in susceptible hosts.