EXPRESSION OF THE MAJOR CORE ANTIGEN VP7 OF AFRICAN HORSESICKNESS VIRUS BY A RECOMBINANT BACULOVIRUS AND ITS USE AS A GROUP-SPECIFIC DIAGNOSTIC REAGENT
EXPRESSION OF THE MAJOR CORE ANTIGEN VP7 OF AFRICAN HORSESICKNESS VIRUS BY A RECOMBINANT BACULOVIRUS AND ITS USE AS A GROUP-SPECIFIC DIAGNOSTIC REAGENT
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DOI：
10.1099/0022-1317-73-4-925
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发表时间：
1992-04-01
影响因子：
3.8
通讯作者：
ROY, P
中科院分区：
文献类型：
--
作者：
CHUMA, T;LEBLOIS, H;ROY, P
非洲马病病毒血清型4(AHSV-4)的主要核心蛋白VP7在昆虫细胞中表达，该病毒是最近在南欧爆发的非洲马病病毒的病原体。重组杆状病毒含有相关AHSV基因的克隆拷贝(S7)。对其生化和抗原性的分析证实了表达的蛋白质的真实性。苜蓿夜蛾核型多角体病毒强多角体启动子控制下VP7的高效表达在感染昆虫细胞中诱导盘状晶体。这使我们能够通过一步超速离心法提纯该蛋白，并将其用于检测马对各种AHSV血清型的抗体。AHSV与其他两种环状病毒--蓝舌病毒和流行性出血性疾病病毒--的血清学关系也被证明。
The major core protein, VP7, of African horsesickness virus serotype 4 (AHSV-4), the aetiological agent of a recent outbreak of the disease in southern Europe, was expressed in insect cells infected with a recombinant baculovirus containing a cloned copy of the relevant AHSV gene (S7). Analyses of its biochemical and antigenic properties confirmed the authenticity of the protein expressed. The high-level expression of VP7 under the control of the strong polyhedrin promoter of Autographa californica nuclear polyhedrosis virus induced disc-shaped crystals in infected insect cells. This enabled us to purify the protein by a one-step ultracentrifugation procedure and to utilize it for the detection of antibodies raised in horses to various serotypes of AHSV. A serological relationship between AHSV and two other orbiviruses, bluetongue virus and epizootic haemorrhagic disease virus, was also demonstrated.