Centromere tethering confines chromosome domains.
Centromere tethering confines chromosome domains.
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DOI：
10.1016/j.molcel.2013.10.021
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发表时间：
2013-12-26
期刊：
影响因子：
16
通讯作者：
Bloom K
中科院分区：
文献类型：
--
作者：
Verdaasdonk JS;Vasquez PA;Barry RM;Barry T;Goodwin S;Forest MG;Bloom K
染色体的区域组织在广泛的细胞过程中起着重要作用，包括基因表达，转录和DNA修复。目前的理解在很大程度上排除了染色质聚合物的时空动态波动。我们结合联合收割机在体内染色质运动分析与数学建模，阐明的物理性质，形成和波动的领土。染色体运动沿着染色体长度方向以可预测的方式变化，这取决于着丝粒处的系留。在着丝粒失活时系链的脱离导致空间移动性增加。两端系有约束珠弹簧链提供了一种机制，以产生所观察到的局部移动性变化，作为与系绳距离的函数。这些预测是实现在实验确定的更高的有效弹簧常数接近着丝粒。染色体的动态波动和区域组织，在一定程度上是由着丝粒的束缚决定的。
The organization of chromosomes into territories plays an important role in a wide range of cellular processes including gene expression, transcription, and DNA repair. Current understanding has largely excluded the spatio-temporal dynamic fluctuations of the chromatin polymer. We combine in vivo chromatin motion analysis with mathematical modeling to elucidate the physical properties that underlie the formation and fluctuations of territories. Chromosome motion varies in predicted ways along the length of the chromosome, dependent on tethering at the centromere. Detachment of a tether upon inactivation of the centromere results in increased spatial mobility. A confined bead-spring chain tethered at both ends provides a mechanism to generate observed variations in local mobility as a function of distance from the tether. These predictions are realized in experimentally determined higher effective spring constants closer to the centromere. The dynamic fluctuations and territorial organization of chromosomes are, in part, dictated by tethering at the centromere.