Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy
Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy
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DOI：
10.1038/jcbfm.2012.42
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发表时间：
2012-06-01
影响因子：
6.3
通讯作者：
Chen, You-Yin
中科院分区：
文献类型：
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作者：
Liao, Lun-De;Lin, Chin-Teng;Chen, You-Yin
总血红蛋白浓度（HbT）、脑血容量（CBV）和血红蛋白氧饱和度（SO2）变化的光学成像提供了研究脑血流动力学调节的手段。然而，高分辨率经颅成像仍然具有挑战性。在这项研究中，我们采用了一种新的功能性光声显微镜技术，以探测单一的皮质血管的反应，左前爪电刺激小鼠完整的头骨。在上级矢状窦和大脑前动脉系统的不同大小的小动脉中的HbT、CBV和SO 2的功能变化以清晰的36 × 65 μ m（2）空间分辨率进行双侧成像。此外，在激活过程中，单个血管中SO2的早期减少（即，“初始浸渍”）。我们的研究结果表明，最初的下降特别发生在小动脉的激活区域，但不是在大静脉。这项技术补充了其他现有的成像方法的调查血液动力学反应，在单一的脑血管。Journal of Cerebral Blood Flow & Metabolism（2012）32，938-951; doi：10.1038/jcbfm.2012.42; 2012年4月4日在线发表
Optical imaging of changes in total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2) provides a means to investigate brain hemodynamic regulation. However, high-resolution transcranial imaging remains challenging. In this study, we applied a novel functional photoacoustic microscopy technique to probe the responses of single cortical vessels to left forepaw electrical stimulation in mice with intact skulls. Functional changes in HbT, CBV, and SO2 in the superior sagittal sinus and different-sized arterioles from the anterior cerebral artery system were bilaterally imaged with unambiguous 36x65-mu m(2) spatial resolution. In addition, an early decrease of SO2 in single blood vessels during activation (i.e., 'the initial dip') was observed. Our results indicate that the initial dip occurred specifically in small arterioles of activated regions but not in large veins. This technique complements other existing imaging approaches for the investigation of the hemodynamic responses in single cerebral blood vessels. Journal of Cerebral Blood Flow & Metabolism (2012) 32, 938-951; doi:10.1038/jcbfm.2012.42; published online 4 April 2012