Analysis of differential gene expression by bead-based fiber-optic array in growth-hormone-secreting pituitary adenomas
Analysis of differential gene expression by bead-based fiber-optic array in growth-hormone-secreting pituitary adenomas
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DOI：
10.3892/etm.2010.137
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发表时间：
2010-09-01
影响因子：
2.7
通讯作者：
Zhang, Yazhuo
中科院分区：
文献类型：
--
作者：
Jiang, Zhiquan;Gui, Songbo;Zhang, Yazhuo
生长激素分泌垂体腺瘤（GHomas）约占所有垂体肿瘤的20%。然而，瘤的发病机制仍有待阐明。为了探讨ghoma的可能发病机制，我们使用基于脑珠的光纤阵列检测了5个ghoma的基因表达，并将它们与3个健康垂体进行了比较。随机选取4个差异表达基因进行实时定量逆转录聚合酶链反应验证。然后，我们使用京都基因和基因组百科全书对鉴定的差异表达基因进行通路分析。阵列分析显示，353个基因和206个表达序列标签（est）表达显著增加，565个基因和29个est表达显著减少。生物信息学分析表明，HIGD1B、HOXB2、ANGPT2、HPGD和BTG2基因可能在GHomas的发生和发展中发挥重要作用。通路分析表明，无翼型信号通路和细胞外基质受体相互作用可能在胶质瘤的发生和发展中起关键作用。我们的数据表明，有许多异常表达的基因和途径参与了肿瘤的发病机制。基于微球的光纤阵列结合差异表达基因的通路分析似乎是研究肿瘤发病机制的有效方法。
Growth-hormone-secreting pituitary adenomas (GHomas) account for approximately 20% of all pituitary neoplasms. However, the pathogenesis of GHomas remains to be elucidated. To explore the possible pathogenesis of GHomas, we used bead-based fiber-optic arrays to examine the gene expression in five GHomas and compared them to three healthy pituitaries. Four differentially expressed genes were chosen randomly for validation by quantitative real-time reverse transcription-polymerase chain reaction. We then performed pathway analysis on the identified differentially expressed genes using the Kyoto Encyclopedia of Genes and Genomes. Array analysis showed significant increases in the expression of 353 genes and 206 expressed sequence tags (ESTs) and decreases in 565 genes and 29 ESTs. Bioinformatic analysis showed that the genes HIGD1B, HOXB2, ANGPT2, HPGD and BTG2 may play an important role in the tumorigenesis and progression of GHomas. Pathway analysis showed that the wingless-type signaling pathway and extracellular-matrix receptor interactions may play a key role in the tumorigenesis and progression of Gliomas. Our data suggested that there are numerous aberrantly expressed genes and pathways involved in the pathogenesis of GHomas. :Bead-based fiber-optic arrays combined with pathway analysis of differentially expressed genes appear to be a valid method for investigating the pathogenesis of tumors.