PDGFBB-modified stem cells from apical papilla and thermosensitive hydrogel scaffolds induced bone regeneration
PDGFBB-modified stem cells from apical papilla and thermosensitive hydrogel scaffolds induced bone regeneration
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DOI：
10.1016/j.cbi.2019.108931
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发表时间：
2020-01-25
影响因子：
5.1
通讯作者：
Liu, Yuehua
中科院分区：
文献类型：
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作者：
Deng, Jiajia;Pan, Jie;Liu, Yuehua
由癌症手术或创伤引起的骨缺损对人类健康具有强烈的负面影响。用细胞和基于材料的复合物治疗为受损骨组织的再生提供了一种替代策略。温敏性水凝胶具有良好的物理性能和生物降解性，是一种很有价值的支架材料。血小板源性生长因子BB（plateletderivedgrowthfactorBB，PDGFBB）主要由血小板分泌，促进间充质干细胞（mesenchymalstemcells，MSCs）的迁移和血管生成。虽然已知PDGFBB间接增强体内骨修复，但PDGFBB对来自根尖乳头的干细胞（SCAPs）的影响需要进一步研究。本研究采用细胞计数试剂盒-8和活/死染色法检测细胞增殖情况。结果表明，PDGFBB可促进SCAPs的增殖。实时荧光定量PCR和Western blot检测成骨相关基因和蛋白。此外，在Sprague-Dawley大鼠中创建颅骨缺损并植入不同的复合物。显微CT和苏木精-伊红分析结果表明，与其他三组相比，水凝胶与慢病毒上清液-绿色荧光蛋白-PDGFBB组合显著改善新骨形成和矿化。总之，我们的研究表明，热敏性水凝胶可用作三维细胞培养的支架，PDGFBB基因修饰的SCAPs可改善颅骨缺损中的骨形成。
Bone defects caused by cancer surgery or trauma have a strong negative impact on human health. Treatment with cell and material-based complexes provides an alternative strategy for the regeneration of damaged bone tissue. The good physical properties and suitable biodegradability of a thermosensitive hydrogel has been shown to act as a valuable scaffold. Platelet derived growth factor BB (PDGFBB) is mainly secreted by platelets and promotes the migration and angiogenesis of mesenchymal stem cells (MSCs). Although PDGFBB is known to indirectly enhance bone repair in vivo, the effects of PDGFBB on stem cells from apical papilla (SCAPs) require further investigation. In our study, the proliferation of cells was investigated by the cell counting kit-8 and live/dead staining methods. The results indicated that PDGFBB promoted the proliferation of SCAPs. Real-time polymerase chain reaction and Western blot experiments were used to detect osteogenic genes and proteins. Moreover, calvarial defects were created in Sprague-Dawley rats and different complexes implanted. The results shown by micro-CT and hematoxylin and eosin analysis demonstrated that the hydrogel combined with lentiviral supernatant-green fluorescent protein-PDGFBB significantly improved new bone formation and mineralization compared with the other three groups. In summary, our research showed that a thermosensitive hydrogel can be used as a scaffold for 3D cell culture, and PDGFBB gene-modified SCAPs can improve bone formation in calvarial defects.